The Mechanism Of Sox9-mediated Wnt/β-Catenin Signaling Pathway Affects The Cutaneous Squamous Cell Carcinoma Cell Proliferation

Objective:To investigate the mechanism of Sox9-mediated Wnt/β-catenin signaling pathway affects the cutaneous squamous cell carcinoma cell proliferation.Methods:1. SCC12 cells( human cutaneous squamous cell carcinoma cell line) are subcultured for experimental research. Synthesis of adenovirus as vector, the experiment group is adenovirus mediated mi RNA transfection group, while control group is empty vector group. Transfection efficiency are assayed after transfection.2. The expression of β-catenin genes after adenovirus transfection is detected by western blotting. The proliferation of SCC12 cells after adenovirus-mediated miRNA silent β-catenin is observed by colony formation assay.3. The expression of Sox9 genes after adenovirus-mediated miRNA silentβ-catenin is detected by western blotting.4. The proliferation of SCC12 cells after adenovirus-mediated miRNA silent Sox9 is observed by colony formation assay.5. The overexpression of Sox9 genes is applied by adenovirus-mediated Sox9 after adenovirus-mediated miRNA silent β-catenin. The relationship betweenβ-catenin and Sox9 in squamous cell carcinoma is investigated by colony formation assay.Results:1. The proliferation of SCC12 cells of silent β-catenin gene decrease, which is statistical significance compared with the Scr control group(P<0.05).2. The expression of Sox9 genes after adenovirus-mediated mi RNA silentβ-catenin decrease significantly.3. The expression of Sox9 genes after adenovirus-mediated miRNA silent Sox9 inhibits the proliferation of SCC12 cells significantly, which is statistical significance compared with the Scr control group(P<0.05).4. The overexpression of Sox9 genes in SCC12 cells of silent β-catenin genes,the proliferation of SCC12 cells partially restored, which is statistical significance compared with the Scr control group(P<0.05).Conclusion:The proliferation of SCC12 cells is inhibited after application of adenovirus-mediated miRNA β-catenin in squamous cell carcinoma SCC12 cells. Our results suggest that Sox9 gene is a transcription factor, regulating by β-catenin, and Sox9 gene can promote the proliferation of SCC12 cells.