| Objective:Flying high load environments(+ Gz) and weight loss environment is an important factor in the pilot disc degeneration(degenerative disc disease, DDD) leads. This experiment simulated the establishment of high load and weightless environment of New Zealand white rabbits spine imaging and intradiscal matrix metalloproteinase(mateixmetalloproteinases, MMP) and tissue inhibitorofmetalloproteinase(TIMP) content changes were observed and analyzed. To investigate the high loads and weightless environment interaction effects on disc, in order to provide experimental and theoretical basis for the prevention of delayed pilot lumbar disc degeneration..Methods:Select an average weight of 120 3KG, 7-8 months, healthy male New Zealand white rabbits, all animals reared under the same conditions, feeding, using the remainder were randomly divided into four groups. Both in the control group, lost reorganization, high load group, high load / weight loss mixed groups, each subdivided into 30 days, 60 days, 90 days three subgroups. Imaging using X-ray, CT, MRI observation, which is lateral X-ray observation of animals whether to change the physiological curvature of the spine, vertebrae whether there is slippage, if there is disc space narrowing. CT observing animals lumbar sacral 1 6 whether calcification, with or without spinal stenosis. MRI observation of experimental animals lumbar sacral 1 6 cases of spinal cord compression, and intervertebral disc if there is a change. Thompson method and using the results of classification. Animal experiments using simulated high load centrifuge, 7Gz exposed 60 seconds, repeated three times, each time interval of one day experiment. After the simulated weightlessness using our group improved tail suspension method, using a five-day suspension in each group, the interval two days to restore the natural position, were suspended again after the experiment. Each group were 30 days, 60 days, 90 days after air embolism using experimental animals were killed, the whole lumbar spine animals removed and fixed in formalin select a lumbar sacral 6 as the observed object detection MMP1, MMP3 and TIMP1 expression rate in the disc, the experimental data expressed as mean ± standard deviation; using analysis of variance, and significant differences between the control group at each time period of each comparison between the experimental group, P <0.05 considered statistically significance.Result:(1) Imaging: Through experimental animals in each group X-ray, CT, MRI were compared, in which no significant difference between the groups X-ray and CT imaging results. MRI can be observed high load / weight loss mixed group of 90 animals on day six lumbar sacral 1 disc at a certain lower T2 plus privileges, there is a certain decrease in water content, Modic changes occur. The rest of the group did not change significantly.(2) Immunohistochemistry: The animals taken six lumbar sacral 1 disc specimens were MMP1, MMP3 and TIMP1 staining in MMP1, MMP3 and expression within each group TIMP1 disc by immunohistochemical detection chemistry at 400 X under an optical microscope, MMP1, MMP3 and positive TIMP1 appear as brown granules, and mainly distributed in the cytoplasm. MMP1, MMP3 and TIMP1 were less expressed in the selected samples of the control group, the difference was not statistically significant(P> 0.05), the experimental group MMP1, MMP3 and both appear TIMP1 expression(P <0.05), in which the high load / weightlessness mixed group most obvious expression in each time period, high load group, followed by loss of the recombinant expression rate in the same time period in each group minimum..Conclusion: High load / weight loss will cause the animal lumbar disc degeneration, both of which can lead to disc MMP1, MMP3 and TIMP1 content changes, and with the extension of the degeneration of the time and more apparent. Including high load and degeneration weightlessness mixed group of the most obvious, simple high load, followed by the final loss for the simple restructuring. TIMP1 and early changes in each group that is obvious, high load / weight loss group 90 days post-reduced sensitivity. Therefore, this kind of change is considered one of the causes of disc degeneration. The results suggest that a high load / weight loss can lead to MMP and TIMP content changes, and the interaction between the two can be accelerated disc degeneration..Background: In recent years, artificial cervical disc replacement surgery as a new method for the treatment of cervical disease gradually been accepted and understood, but it is also associated with complications caused by progressive scholars attention.Objective: To investigate the activity of artificial cervical disc replacement clinical effects of the treatment of cervical disease and prosthesis.Method:Select our department in August 2006 in April 2012, 25 cases of the use of artificial cervical disc replacement in the treatment of cervical spondylosis, including 15 males and 10 females, including six cases of double segment, single segment 19 cases, aged 31-76-year-old, the average age of 51.04 years old up for 24-93 months, with an average 48.96 months, the clinical results using the Japanese Orthopaedic Association(Japanese Orthopaedic Association, JOA) score, cervical dysfunction index(neck disabrlity index, NDI), pain visual analog(visual anatague scale, VAS) scores were assessed using the activity of the surgical segment imaging observations, cervical curvature, heterotopic ossification, and degeneration of adjacent series of changes etc. segments.Results:All patients with neurological varying degrees of improvement, one case of mild heterotopic ossification, but no clinical symptoms; no significant difference between preoperative surgical segment of the festival, up and down the activity of adjacent segments last follow-up(P> 0.05); no C2 ~ C7 activity when C2 ~ C7 activity preoperative and final follow-up statistical difference(P> 0.05); former JOA score surgery(12.4 ± 1.4) points, last follow-up(15.0 ± 1.4) points, the difference was statistically significant(P <0.05); improvement rate was 51%; VAS score before surgery(5.4 ± 2.1) points, last follow-up(1.1 ± 1.0) points, the difference was statistically significant(P <0.05); NDI score before surgery(25.5 ± 8.0) points, last follow-up(6.6 ± 4.6) points, the difference was statistically significant(P <0.05); Conclusion artificial cervical disc replacement in the treatment of cervical disease can achieve better clinical efficacy, while You can keep the activity replacement segment avoid accelerated degeneration of adjacent segments.Conclusions: Artificial cervical disc replacement in the treatment of cervical disease can achieve better clinical efficacy, while maintaining the activity of the replacement segment, avoiding the accelerated degeneration of adjacent segments. |
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