The Expression Of PKM2in Intrahepatic Cholangiocarcinoma And Its Carcinogenic Effect

Background:Intrahepatic cholangiocarcinoma is an aggressive malignancy and is one of the most devastating cancers of the gastrointestinal tract. The molecular mechanisms contributing to the pathogenesis of these cancers are not well understood. Pyruvate kinase type M2(PKM2) has been reported to be involved in aerobic glycolysis and cell growth in various tumors. However, the expression pattern of PKM2in intrahepatic cholangiocarcinoma (ICC) and the correlation between PKM2expression and ICC remains unclear. The aim of this study is to investigate PKM2expression and its possible role in ICC.Methods:50cases of intrahepatic cholangiocarcinoma tissues and matched normal adjacent tissues were collected. The expression of PKM2was detected by immunohistochemistry and RT—CR, and the relationship between the expression of PKM2and overall survival of was analyzed. In in vitro experiments, PKM2was knocked down using a siRN A, and tumor cell behavior were analyzedResults:We found that expression of PKM2was increased in ICC (Relative mRNA:3.24±0.693VS0.48±0.109, P<0.001), and the increased PKM2expression was associated with overall survival of the ICC patients(immunohistochemistry score:40.61±4.537(score≤7) VS15.15±2.368(score>7), P<0.001). Knockdown of PKM2repressed proliferation, invasion and migration of the9810and CCLP-1cells.Conclusion:Results suggest that the expression of PKM2plays a critical role in development of ICC, and it may provide a growth advantage for ICC cells. Thus, PKM2might be a potential therapeutic target for ICC.