Study On Specificity Quality Evaluation Of A Tractylodis Lancea Bran Fried Before And After Based On The Principle Of Processing

Rhizoma Atractylodis of compositae Rhizoma Atractylodis Atractylodes lancea(Thunb.) DC. Or north Rhizoma Atractylodis Atractylodes Chinensis Koidz. Dried roots, is a perennial herbaceous plant. Raw Rhizoma Atractylodis temperature drying and tangy, bran Fried modifiable Rhizoma Atractylodis drying, enhance the effect of the spleen and stomach. National natural fund project studies of bran Fried Rhizoma Atractylodis processing mechanism is the material base is studied to improve effect and decrease dry, clear the influence effect of chemical composition. This paper based on the early research team, on the basis of the existing standard of "pharmacopoeia of the contrast of raw Rhizoma Atractylodis quality standard, the bran Fried Rhizoma Atractylodis specificity quality evaluation index. Bran fried Rhizoma Atractylodis for exclusive quality evaluation; make clear on the efficacy, a significant amount of 5-HMF determination and transformation mechanism, specifically raw bran fried Rhizoma Atractylodis and content differences 5-HMF, and analysis process on bran fry 5-HMF influence content, to further explain the process mechanismTo explore the chemical composition of Rhizoma Atractylodis slices bran Fried designed properties before and after changes, methyl siloxane as stationary phase, The carrier gas: He(99.999%); Injection port temperature; 230 ℃, split ratio 10:1; FID detector temperature: 240 ℃, the tail gas, N2(99.999%), 1μl sample quantity, carrier gas flow rate 1.3 ml/min, carrier gas ratio Air: H2: N2 = 350: 35:35, Temperature programmed for 10 batches of raw rhizoma atractylodis and corresponding bran Fried slices of Rhizoma Aractylodis slices n-hexane extracts of gas chromatography, Found the ratio of 65.620 min chromatographic peak area and the 68.13 min chromatographic peak area in raw Rhizoma Atractylodis slices were greater than 2. n the bran Fried Rhizoma Atractylodis slices were less than 2, methodology to investigate stability, can be used as a raw Rhizoma Atractylodis and bran Fried the identification features of Rhizoma Atractylodis.Rhizoma atractylodis yinpian bran fry feature maps in liquid phase change law before and after the study,Octadecyl silane bonded silica as filler, Mobile phase of acetonitrile, water, flow rate of 1.0 mL /min, detection wavelength of 254 nm, column temperature 30 ℃, 20 Mobile phase of acetonitrile, water, flow rate of 1.0 mL min- 1, detection wavelength of 254 nm, column temperature 30 ℃, 20μl of incoming sample amount is of incoming sample amount is, Gradient elution of 10 batches of raw Rhizoma Atractylodis and corresponding bran Fried slices of Rhizoma Atractylodis slices n-hexane extracts of high performance liquid chromatography(HPLC) analysis, The results showed that the ratio of 46.89 to min chromatographic peak area and 45.48 min peak area greater than 1 in raw Rhizoma Atractylodis slices, In the bran Fried Rhizoma Atractylodis slices were less than 1;but the ratio of 51.22 min chromatographic peak area and 48.69 min peak area less than 1, In the bran Fried Rhizoma Atractylodis slices were greater than 1. Difference law, can the qualitative identification of Rhizoma Atractylodis and bran Fried Rhizoma Atractylodis, specificity.The early stage of the team through the comparative study of Rhizoma Atractylodis bran Fried chemical composition before and after separation of 5- HMF to affect one of the ingredients.Octadecyl bonded silica as filler, Mobile phase: methanol- water solution(95:5) flow rate: 1.0 mL, min- 1; : detection wavelength of 284 nm; Column temperature: 30 ℃; Sample quantity 20μl, Raw Rhizoma Atractylodis and bran Fried slices of 5 and HMF content in Rhizoma Atractylodis slices determination found bran Fried 5- HMF content in Rhizoma Atractylodis slices than raw Rhizoma Atractylodis increased 10 times in the slices, cut stable trend, significant differences between them. Results 5- content in raw rhizoma atractylodis slices were greater than0.01mg/gless than 0.05mg/gsults and bran Fried in rhizoma atractylodis 5- content were higher than 0.12mg/g.To clear under the condition of Rhizoma Atractylodis slices bran Fried technology to produce 5- HMF principle, through consulting literature and experimental research, found that fructose is the conversion of the physical and chemical reaction in the process of TCM firing 5-HMF increases the main way. Transformation is the main condition under the condition of heating, a molecule of fructose took off three water molecules. Octadecyl bonded silica as filler, Column temperature 30 ℃. With acetonitrile- water(2:98) as mobile phase, flow rate of 0.6 ml/min. Sample volume 1μl. ELSD not bypass mode, the drift tube temperature 110 ℃, carrier gas flow rate of 3.1 L/ml, Raw Rhizoma Atractylodis and bran Fried slices Rhizoma Atractylodis slices testing found that Rhizoma Atractylodis bran Fried slices and contains a lot of fructose, and fructose content in raw Rhizoma Atractylodis slices is bran Fried in Rhizoma Atractylodis slices, stable content changes. The fructose variation with 5-HMF results after conversion, bran Fried 5- content, has significantly stability.This experiment based on the principle of Rhizoma Atractylodis bran Fried research, choose quality, bran Fried Rhizoma Atractylodis, the characteristics of bran Fried significantly before and after chemical into or ingredients group of evaluation indexes for quality, quality evaluation content clearly, To perfect quality evaluation indexes before and after Rhizoma Atractylodis bran Fried slices made important supplement.