| Background: All along, lung cancer is one of the major problems that affects human health. Therefore, it is extremely important to elucidate the pathogenesis of lung carcinoma and find a effective treatment of patients with cancers. S100A8 and S100A9 belong to the family of S100 calcium-binding protein, the expression of whom is variance in malignancies. Current research data shows that, the expression of S100A8/A9 is up-regulated in the most of abdominal and pelvic tumors such as gastric cancer, pancreatic cancer, liver cancer, colorectal carcinoma, bladder tumor, cervical cancer and the breast cancer of females, while it is down-regulated in laryngeal carcinoma. Therefore, some scholars suggested that S100A8 and S100A9 has the dual roles of anti-tumor and pro-tumor during the genesis and development of malignant tumors, and their effect is concentration dependent. At their higher concentrations(20-250 μg/ml), S100A8 and S100A9 exert its anti-tumor effect by promoting tumor cell apoptosis. While at their lower concentration(<20 μg/ml), they play its role of pro-tumor by promoting cancer cell proliferation. And some studies have demonstrated that the effect of anti-tumor is not dependent on RAGE, but its role of pro-tumor is mediated by RAGE meanwhile the MAPK and NF-κB are activated. Besides, the large number of data shows that the expression of some S100 family members such as S100A2, S100A4 and S100A6 is increased in the the cells of lung cancer. Recently, A tole of 49 up-regulated expression proteins including S100A8 and S100A9 are identified in the human lung squamous carcinoma compare with normal bronchial epithelium using the 2D-LC-MS/MS by Xu Yan and her partners. But they only show the expression of S100A8 and S100A9 is up-regulated in lung squamous carcinoma, not to describe the characteristics of S100A8 and S100A9 expression in lung cancer and their relationships with the clinical data detailedly.Purpose: To investigate the characteristics of S100A8 and S100A9 expression in the lung adenocarcinoma and squamous carcinoma and their correlation with the clinical data such as gender, age, TNM stage, etc.Methods: 20 pairs of non small cell lung cancer and adjacent normal tissues were collected from the Department of Thoracic Surgery, Huaihe Hospital affiliated Henan University during the January 2013 to December 2014. Besides, 100 paraffin samples diagnosed with lung adenocarcinomas and squamous carcinoma and 20 paraffin samples of normal tissues during the January 2007 to December 2009 were obtained from the Department of Pathology. The PCR, western Blot and immunohistochemical staining were used to investigated the expression of S100A8 and S100A9 in cancer tissues and adjacent normal tissues, and to analyze its correlation with the clinical data.Results:(1)The PCR and western blot showed a significant up-regulation of S100A8 and S100A9 expression in cancer tissues of non small lung cancer(P<0.001).(2) Immunohistochemical staining showed that, the S100A8 and S100A9 were expressed in the both cytoplasm and nucleus, but mainly expressed in the cytoplasm. And the expression of both proteins was identified significant up-regulation in cancer tissues(P<0.05).(3)The expression of S100A8 was no difference in the patients of different age, gender and TNM stage(P>0.05). The expression of S100A9 was no difference in age and gender(P>0.05), but it in Ⅰstage was significantly lower than Ⅱ~ Ⅳstage(P<0.001).(4)The expression of both proteins in well-differentiated was identified significantly lower than poor-differentiated adenocarcinoma tissues(P<0.01), while it was not different between well-differentiated and poor-differentiated squamous cell carcinoma(P>0.05).Conclusion: The expression of S100A8 and S100A9 was up-regulated in the cancer tissues of lung adenocarcinoma and squamous cell carcinoma.Moreover that overexpression of the both proteins is associated with a poor-differentiation in lung adenocarcinoma. Besiedes the up-regulation of S100A9 is related to a late TNM stage. Therefore, they could serve as a marker identifying patients of lung adenocarcinoma and squamous cell carcinoma who were at high risk. |
PDF Full Text Request