| Objective 1 Stereotactic method using 6-hydroxy-dopamine(6-OHDA) injection into the striatum of rat substantia nigra of Parkinson’s disease in rats prepare to observe the effect on the behavior of Guiling pa’an pill rat model of Parkinson’s Disease. 2 Determination Guiling pa’an pill on rat model of Parkinson’s disease striatum of glutathione(GSH), glutathione transferase-S(GSH-ST), glutathione peroxidase(GSH-PX) each impact of changes in indicators. 3 Discussion Guiling pa’an pill possible mechanism of oxidative stress; provide a solid scientific basis for the theory Guiling pa’an pill to treat Parkinson’s disease, clinical applications, and achieved a certain degree of economic and social benefits.Method 1 The successful model of 42 rats PD use Excel software completely randomization medicine into high, medium and low dose group, Madopar group and model control group, 5 groups, including nine high-dose group, middle dose group 9, low-dose group 8, 8 Madopar group, model control group eight. Another sham group 10. Analyzing the success of the second day after modeling oral gavage at the end of 30 days, eight high-dose group, middle dose group 8, low-dose group 7, 8 Madopar group, model control group 7, sham group 8.The rats were successful model after the second day orally, twice a day, a total of gavage for 30 days, in which high-dose group administered by 3.75 g / kg / d; middle dose group were 1.87 g / kg / d administration; low dose administered by 0.93 g / kg / d; model group were given the same volume of saline per day; the sham group received the same volume of saline per day. 2 Determination of model behavior: 1,2,3,4 weeks after surgery were performed on all animals apomorphine(0.25mg/kg) induced rotational behavior in rats identification. Injected 10 min after the start of counting, were recorded 40 min. 3 Oxidative stress tests: blood after the animals were killed, quickly separatingand removing the striatum, a 10% saline brain tissue homogenates, with a low centrifuge 3000 rev/min, centrifuged for 15 min, the supernatant, Determination of rat brain tissue GSH, GSH-PX and GSH-ST reagent according to the requirements of the indicators.Result After the two-point injection damaged the left side of the nigrostriatal modeling four weeks, 100 rats in 42(42%) rat model was successful, and the other 10 sham group. After modeling, compared with the model group, sham group did not appear involuntary movements(P<0.05), and the remaining four groups involuntary movements were reduced(P<0.05), with Madopar group the most significant(P<0.05). During treatment, with the model group, Madopar group and Guiling pa’an pill large, medium and small dose group compared with the sham group did not appear rotational behavior, Madopar group and Guiling pa’an pill large, medium and small dose group apomorphine-induced rotation frequency was significantly reduced(P<0.05), with Madopar group is more significant(P<0.05). After the end of treatment, compared with the model group, all damaged striatum GSH, GSH-PX, GSH-ST higher levels(P<0.05).Conclusion 1.Number of turns on the seventh day rotation in rats Ling Ko pill small turtle and tortoise Ling Ko dose pill dose group had no reduction after treatment than before treatment with the drug may be absorbed, distributed, plasma concentration, turtle Ling Wan Ko Department of Chinese medicine, low-dose pill turtles Ling Ko can not quickly achieve effective blood concentration, may be its failure to significantly reduce the number of early PD rats rotation mechanism. Guiling pa’an pill dose group significantly reduced the number of turns in the rotation before the seventh day after treatment than before treatment in rats may be related to the large turtle Ling Ko dose pills can quickly achieve effective blood concentration. In short Guiling pa’an pill can significantly reduce the number of rotations of Parkinson’s disease in rats by apomorphine-induced onset time and Guiling pa’an pill dose-related. 2.within each group rat brain homogenates glutathione, glutathione peroxidase and glutathione-S transfer relatively enzyme content can be found, the Guiling pa’an pill small doses, in dose, high dose group substantia nigra- striatum GSH, GSH-PX, GSH-ST levels increased significantly(P<0.05). Description improve Parkinson’s disease striatum GSH, GSH-PX, GSH-ST content may be one of the Guiling pa’an pill,Madopar play a therapeutic role. 3.Parkinson’s disease rat substantia nigra- striatum GSH content, middle dose group, no significant difference in the high-dose group turtle Ling Ko low dose pill. Substantia nigra- striatum GSH-PX was no significant difference(P>0.05) in the low-dose pill tortoise and antelope Hpa-dose group. Tip Guiling pa’an pill dose or low-dose rat substantia nigra- striatum GSH-PX content on the difference is very small. Guiling pa’an pill dose group and middle-dose group compared with Parkinson’s disease can significantly increase GSH-PX content in rat striatum. Guiling pa’an pill small doses, dose and high-dose group compared with Madopar group of Parkinson’s disease can be significantly increased GSH-PX content in rat striatum. Large doses of Guiling pa’an pill can significantly increase the rat substantia nigra in Parkinson’s disease- striatum GSH-ST content and superior dose, low-dose pill turtles Ling Ko. Ko Wan Ling turtle small doses, dose and high-dose group compared with Madopar group of Parkinson’s disease can be significantly increased GSH-ST content in rat striatum. About Guiling pa’an pill dose pills and Parkinson’s disease substantia nigra- striatum GSH, GSH-PX, GSH-ST content relationship needs further study. |
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