Control Experimental Research On Antagonism Sediment Between Bile Duct Stent With Na-no Sliver Coating And Teflon Bile Duct Stent

Objective: To investigate the feasibility of the silver nano particles coating biliary stents preparation by the chemical oxidation reduction and physical magnetron sputtering respectively, and physical and chemical properties and stability of silver nano particles coating. To establish the obstructive jaundice beagle animal experimental model performing the control experiment for antagonism of stents sediment in vivo,between Teflon biliary stents and silver nano particles coating biliary stents, and to provide animal experiment basis for clinical application.Methods: The experiment is divided into two parts. The first part use the chemical oxidation reduction of silver nano particles coating prepared by the bile duct stent, detection of bile duct stent using scanning electron microscopy(SEM), energy spectrum analysis of silver nano particles coating thickness and chemical stability. The second part of the animal experiment. By carrying out the experiment of 12 with healthy beagle as the research object, after establishing animal models of obstructive jaundice were randomly divided into 2 groups, respectively bile duct carotid stenting. Silver nano particles coating bile duct stent group(n = 6), the common Teflon bile duct stent(n = 6). Postoperative day 7, line and laboratory blood leukocytes, liver result examination, ELISA method to detect the TNF-α. IL- 6. 21 days after execution of experimental animals, bile duct stent and bile duct wall tissue, line respectively: bile duct wall tissue HE staining pathology examination, scanning electron microscopy(SEM) observation bile duct stent sediment adhesion degree; Ultrasonic vibration remove bile duct stent sediment and sediment weight; Infrared spectrum analysis of bile duct stent sediment composition; X-ray spectrometer(EDS) observation after preoperative na-no silver coating stability.Results: 1.Silver nano particles coating biliary stents preparation was applied to chemical oxidation reduction. Silver nano particles coating thickness between 1.5-6μm were measured by SEM, and a single silver ion(Ag+) component was detected by energy dispersive x-ray analysis(EDXA).2. The obstructive jaundice animal experiment model was established. Before modeling, AgNPs coating biliary stents group compared with Teflon biliary stents group, white blood cell(WBC), alanine aminotransferase(ALT), aspertate aminotransferase(AST), total bilirubin(TBIL), direct bilirubin(DBIL) level comparative differences, there were no statistical significance(P>0.05); concentration of TNF-α and IL-6 comparative differences, there were no statistical significance(P>0.05); and all the indexes can meet the demands of modeling. After modeling, the comparison between 2 groups experiment result was consistent with before modeling.3. Animal studies show that biliary stent implantation, canine model of obstructive jaundice blood leukocyte, liver, IL-6, TNF-α were compared before surgery have decreased significantly. Silver nano particles coating biliary stent in the bile duct inflammation reaction degree, sediment thickness, measuring the weight biliary stents are better than ordinary stent. Infrared Spectrum Analysis(ISA) contrasts sediment composition, biliary stents group sediment compound were composed of bilirubin, cholesterol, bile acid, protein, calcium and other substances..The stability of AgNPs coating was perfect before and after the contrast experiment by EDXA.Conclusion: Silver nano particles coating biliary stents preparation was applied to chemical oxidation reduction, Silver nano particles coating biliary stents can satisfy animal requirements.We established the obstructive jaundice animal experiment model of beagles common bile duct ligation successfully. The success rate was higher, and it can meet requirement about Silver nano particles coating biliary stents antagonism of sediment experiment satisfactorily. The effect of antagonism of biliary stents sediment on Silver nano particles coating biliary stents was obviously better than Teflon biliary stents in animal experiments. The group will further to experimental research, as early as possible used silver nano particles in clinical...