| In recent years, Chinese medicine for acute and critically ill patients in clinical work undertaken, septic shock TCM pathogenesis and treatment of deepening the understanding of proposed foreign warm toxin, causing lack of righteousness, gas consumption shade off, context Stagnation gas phase along the yin and yang are not connected, yang canopy on the inside, not the end of the four temperature dependent, so against the cold as Jue, air-reverse chaos, the owner of the pathogenesis of the gods, and modern medicine for understanding mechanisms of septic shock is consistent. Should be cooling blood detoxification, Yang Yin Sheng Jin Fangxianghuazhuo Resuscitation treatment. This paper intends to clinically effective in the previous premise protective effects of Shenxi Dan septic shock in rats, and to study its mechanism of action related cytokines from the perspective of the other party to the governing law as embodied in the treatment of septic shock The mechanisms to open up the idea to provide a theoretical basis for clinical treatment.Objcetive Watch cool blood blood begin to understand the law on behalf of the party Shen Xi Dan for endotoxin shock, the protective effects of visceral injury in rats and the effect of inflammatory cytokines.Methods Will clean level 60 healthy adult male rats were randomly divided into normal group, model group, Shen Xi Dan group. In addition to the normal group, the rest of the group were given abdominal injection D_ galactosamine(Gal N) and tail vein injection of lipopolysaccharide(LPS) duplicate the endotoxin shock animal models, the BL-420E+ biologicalfunction experiment system through monitoring the change of mean arterial pressure(MAP), serum samples at 0, 2, 6 h, detection of whole blood, serum total bilirubin(TB), lactate dehydrogenase(LDH), aspertate aminotransferase(AST), alanine aminotransferase(ALT) levels; Using enzyme-linked immunosorbent method and detection of TNF alpha, IL-1, IL-4, the expression of IL-6 and IL-10 levels. At 0, 2, 6 h to be put to death in the rat, hematoxylin-eosin(HE) staining and observed under light of rat liver pathological changes of lung tissue. Using statistical software spss19.0 to single factor analysis of variance. Experimental data with mean±standard deviation(`x±s) said, compared with t test between groups, X2 test count data is used to P<0.05 said the difference was statistically significant; Statistics is very significant difference(P<0.01) said.Results 1. Changes of the mean arterial pressure: in the experimental group in the process of normal rats blood pressure generally remain normal, there was a slight drop considering is associated with each blood and experimental trauma. Model group rats after injection of endotoxin, most of the blood pressure in 0.5h began to decline, obvious decline when to 1h(about 40%-50% lower than before the injection of endotoxin), then continue to slow lower volatility; Shen Xi Dan group rats blood pressure trend of change are basically the same with the model group, first rapidly falling down slowly again, but the shift to lower blood pressure time later than the model group, both began to decline in 1 h, significantly lower to 2 h. 2, 6 h time points lower level compared with the model group significantly reduce blood pressure(P<0.05 or P<0.01), while Shen Xi Dan no statistically significant differencebetween group and normal group, showed the Shen Xi Dan to endotoxin shock rats have the effect of the booster. 2. Complete blood content changes: 2h, 6h after injection of LPS, white blood cells(WBC) model group were significantly higher than normal group(all P<0.01); Shen Xi Dan in note 2h and 6h WBC also increased significantly after LPS, meaning there was no significant difference compared with normal group(P>0.05), compared with model group(P<0.05 or P<0.01). PLT in 2h, the rhinoceros with Shen Xi Dan set of model group were not significantly decreased(P>0.05); To 6h, PLT model group was obviously lower than normal group(P<0.05), but significantly lower rhinoceros Dan group has no god, endotoxin significantly higher than the model group(P<0.05).(3) in the rat blood biochemical TB, LDH, AST and ALT content changes: compared with normal group, model group rats serum TB, LDH, AST and ALT levels were significantly higher(P<0.01); God rhinoceros Dan group compared with model group, the serum TB drugs after the intervention of Shen Xi Dan group, LDH, AST and ALT levels rise has the remarkable difference compared with model group(P<0.05, P<0.01). The normal rhino with god Dan group there was no significant difference. 4. The rat serum TNF alpha, IL-1, IL-4, IL-6, IL-10 expression level: compared with normal group, model group rats serum TNF alpha, IL-1, IL-4, the expression of IL-6 and IL-10 levels were significantly higher(P<0.01); Shen Xi Dan group compared with model group, after drug intervention, the Shen Xi Dan group serum TNF alpha, IL-1, IL-4, the expression of IL-6 and IL-10 levels rise compared with model group had significant difference(P<0.05, P<0.01), and normal rhino with Shen Xi Dan there was no significant difference.5. The lung tissue pathology change: Macroscopic observation: normal group, the lung expansion is good, the surface without bleeding; Shock model group, lung volume increases, dark yellow, visible on the surface of the varying degree hyperemia and edema, or see the focal point of bleeding; Shen Xi Dan set of lungs are pale red, surface hyperemia edema different level. Then the lung tissue morphology: different time points(0, 2, 6 h) group normal lung tissue morphological structure is clear, alveolar complete, no inflammatory cell infiltration. Model group 2, 6 hours in rat lung tissue inflammation response: 2 hours in the rat pulmonary capillaries expansion bleeding, vascular cavity with micro thrombosis, thickening of alveolar septa wide, a large number of inflammatory cells infiltration; 6 hours of rat lung tissue structure disorder, alveolar cavities fibrin exudation, part of the alveolar atrophy, a large number of inflammatory cells infiltration. Shen Xi Dan group 2, 6 hours of rat lung tissue inflammation response: 2 hours a small amount of red blood cells can be seen in the pulmonary vascular cavity of rats, pulmonary interstitial structure basic normal, alveolar walls are a small amount of inflammatory exudate, visible light to moderate inflammatory cell infiltration; Within 6 hours visible in the rat lung congestion, alveolar space and pulmonary interstitial inflammatory cells infiltration in the visible. 6. Liver tissue pathology change: Different time points(0, 2, 6 h) group normal liver tissue and liver sinus morphological structure is clear, no inflammatory cell infiltration. Model group 2, 6 hours of rat liver tissue inflammation response: 2 hours in the rat liver cells diffuse necrosis, hepatic sinus structure disorder, collect abbacy visible light to moderate inflammatory cell infiltration; 6hours in the rat liver cells of flake diffuse necrosis, hepatic sinus structure damage, portal area shows a large number of inflammatory cells infiltration. Shen Xi Dan group observation of 2, 6 hours of rat liver tissue inflammation response: 2 hours in the rat liver cells a small amount of diffuse necrosis, hepatic sinus structure disorder, collect abbacy visible light to moderate inflammatory cell infiltration; 6 hours in the rat liver cells dotted necrosis, portal area shows a large number of inflammatory cells infiltrationConclusions 1. The cold blood scattered blood begin to understand Shen Xi Dan have booster function. 2. Shen Xi Dan inhibits leukocyte, AST, ALT, TB, LDH, reduce platelet aggregation, protect liver, improve microcirculation. 3. Shen Xi Dan can significantly reduce endotoxin shock rat lung and liver tissue inflammation. 4. Cool blood scattered blood begin to understand Shen Xi Dan can significantly reduce the rat serum proinflammatory factor and antiinflammatory factor expression level, the inhibition of endogenous endotoxin induced the release of inflammatory mediators, and maintain body temperature stability, to restore the body’s internal normal physiological function. |
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