Study On Quality Characteristics Of Pulsatilla Cernua In Changbai Mountain District

Pulsatilla chinensis is a kind of traditional Chinese medicine, but also a genuine medicinal materials in Changbai Mountain District, with Qingrejiedu, Liangxue antidiarrheal effect. Many domestic Chinese medicinal materials production sale, the source varies from place to place, and when the real estate is the genus Pulsatilla chinensis is consistent, that is the original source of Pulsatilla medicinal plants in the genus when the property. According to Lv Huizi reported in Changbai Mountain area are widely distributed in Xingan Chinese Bulbul (P. dahurica), Pulsatilla chinensis (P.cernua) and Yanbian (P. yanbianensis H.Z.LV) three.This paper studied on three kinds of Pulsatilla chinensis in Yanbian area. The results show that, the three kinds of Pulsatilla microspore development process was similar, Pulsatilla individual pollen sac in tetrads of microspores showed abnormal.The study of Radix Pulsatillae in Yanbian area belongs to anther culture, embryo development and genetic breeding research and to provide a basis.Secondly, using thin layer chromatography. The chemical composition of 10 different areas in Yanbian was conducted a preliminary study on the fingerprint. The results show that, in different areas in Yanbian. The spots on the TLC plate Rf and the same size as the main color, dot is 6; the control and standard, and hederagenin spots are consistent, and Pulsatilla Saponin B4 dot is not only has the same also inconsistent. Therefore, the difference of chemical composition between Yanbian white also has the certain regularity, but the main composition changed greatly, breed characteristics is not obvious, and then we use HPLC method, the establishment of the Yanbian region of Longjing, Helong, reed ditch in 10 different regions of high performance liquid chromatography fingerprint of Radix Pulsatillae,11 common peaks were confirmed and the similarity of 10 different areas in Yanbian. High performance liquid chromatography and calculation showed that have high similarity.Finally, the content of Yanbian area of Longjing, Helong, reed ditch 10 different origin herbs Pulsatilla Saponin B4 by high performance liquid chromatography was analyzed, the content of 10 different areas in Yanbian of Pulsatilla Saponin B4 at 3.25-5.99 mg/g, the content is similar.