The Establishment Of C57 Mouse Model With DVT Stenosis Of Inferior Vena Cava And The The Correlation Between PAR1, VCAM-1 And Deep Venous Thrombosis

Objective1. To establis the C57 mouse DVT model with stenosis of inferior vena cava, andto explore the effect of thrombosis in different degree of stenosis of nferior vena cava in DVT model.2. Harvested the inferior vena cava and thrombus organization of C57 mouse after 24 hours,thrombus was observed by HE staining, the expression of PAR1 and VCAM-1 of venous wall tissue was detected by RT-PCR.Methods1.60 C57 mice were randomely divided into group A ((29G type needle, diameter=0.33mm) and group B 30 (30G type needle, diameter=0.3mm). The two groups were used 29G and 30G insulin needles for stenosis of inferior vena cava blood stasis method in mice model, respectively.Recording the weight and inferior vena cava diameter of two groups in the process of procedure, the diameter of inferior vena cava (marked as D), the radius is D/2 (marked as R), and then calculatehe the cross sectional area (marked as A, A=π r2)of the C57 mouse inferior vena cava; according to the two groups of different diameter of needle (marked d),needle radius d/2 (marked r), calculated the cross sectional area (marked as B, B=π r2,) after procedure;the residual lumen area ratio=the cross sectional area after procedure/the original cross sectional area*100% =B/A*100%;The degree of stenosis=(A-B)/A*100%.set up time points at 24hours (acute DVT in mice)after the procedure,Observe and record the death rate and thrombosis rate at the same time, harvested the inferior vena cava organization,According to the data analysis and compare thestability of two different stenosis rate of thrombosis in inferior vena cava stenosis model.2.120 C57 mice were randomly divided into 3 groups:normal control group 40 rats,40 rats in the sham operation group, and 40 rats in inferior vena cava stenosis group.The normal group feeds normally,the sham operation group exposure the inferior vena cava and suture the abdominal, The DVT model group through a selection needle into a stenosis rate stable model of thrombus in inferior vena cava stenosis method.For 24 hours after the model of thrombosis and venous wall tissue sections and HE staining, observed under light microscope in different times from the micro.Harvested the inferior vena cava from both the normal group and the sham operation group at 24 hours,compare with the DVT group by histological section and HE staining.Using the real-time PCR method,, the changes of PAR1、VCAM-1 expression were measured and compared between the three groups.results1 Model group A (29G type needle diameter=0.33mm, the mortality rate was 6.67%), model group B (30G type needle, diameter=0.3mm) the mortality rate was 3.33%, two groups of death shows no statistically significant (p>0.05).The thrombosis rate of 29G model was 60.71%,The thrombosis rate of 30G model was 86.67%,the two groups shows statistically significant (p<0.05),29G model:the residual stenosis rate was 88.82%, the lumen cross-sectional area ratio was 11.18%; 30G model:the residual stenosis rate was 90.67%, the lumen cross-sectional area ratio was 9.33%.Both datas show statistically significant (p<0.05), The weight of thrombus in two in the model group the difference was statistically significant (p<0.05), the difference between thrombus length in the two model group were not statistically significant (P> 0.05),the body weight and inferior vena cava diameter of C57 mice was positively correlated2. After 24 hours of the procedure with inferior vena cava stenosis model of C57 mice, the hind limbs show swelling, and the activity was limited; black material clot was observed in the inferior vena cava,The inferior vena cava swell significantly; under light microscope:the thrombus filled the lumen, thrombosis and adhere with the most of vein wall.Visible vein wall inflammatory cell infiltration, without the tissue organized。Normal group and the sham group HE staining of the vein wall is smooth, no lumen thrombosis.3.The increased expression of PAR1, VCAM-1 in C57 mice DVT model of vein stenosis of inferior vena cava in the wall, and there was a significant difference compared with the normal control group and sham operation group (P<0.05), there was no statistically significant between normal group and sham operation group (P> 0.05).Conclusion1.Inferior vena cava stenosis can successfully established the mouse model of DVT C573.PAR1, VCAM-1 and deep vein thrombosis is closely related.