| OBJECTIVE: To establish an experiment animal model of the VX2tumor invading IVC and observe its biological behaviour.METHODS: Abdominal cavity was opened, the prepared VX2tumortissue mass was transplanted into the soft tissues around the IVC below thekidney in28rabbits, then the rabbits were randomly divided into group Aand group B。Group A：The size of tumor, the diameter of the IVC and theAA,and the surrounding tissues were detected with the ultrasound once aweek. The collateral circulation was observed with DSA. Group B:4rabbits were randomly dissected weekly, the IVC and the AA were removedfor pathological and immunohistochemical analysis and metastasis in lungkidney, liver and celiac was observed at the same time.RESULTS:1, Ultrasound examination shows: all VX2tumors hadbeen growing successfully in the model rabbits, The tumors’ area weresignificantly positive related to time (r1=0.879，P<0.0001), the diameter ofIVC were significantly positive related to tumors’ area（r2=0.829，P<0.0001）.There were25%lumen of IVC occluded in the first four weeks,and91.7%in the first five weeks. During the observation period thelumen and wall of AA were normal.2, The pathological andImmunohistochemical examination shows: the wall of IVC was graduallyreplaced by tumor cells, and there wasn’t any tumor tissue breakthrough theoutermembrance of the AA.3, DSA examination shows: the collateralcirculation had been developing well when the IVC was gradually invaded.4, Anatomic observation: The tumor metastasized to lung and other organsin3weeks after transplantation.CONCLUSION: The model by implanting VX2tissue mass aroundthe insides of IVC in rabbits is similar to the tumor invading the IVC inhuman body. |
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