The Expression Of BRMS1 And MACC1 In Colorectal Carcinoma And Its Clinical Significance

Objective:To detect the expression of breast cancer metastasis suppressor 1 (BRMS1) and metastasis-associated in colon cancer 1 (MACC1) in colorectal carcinoma tissues and corresponding adjacent normal colorectal tissues. To explore the association of their expression with colorectal clinicopathological characteristics and the correlation of BRMS1 mRNA and MACC1 mRNA in colorectal carcinoma.Methods:30 cases of colorectal carcinoma specimens after surgical resection and pathologically confirmed were collected in the Second Affiliated Hospital of Kunming Medical University from September 2013 to May 2014.Using real-time fluorescence quantitative PCR technique with β-actin gene as internal reference, according to 2"-△△Ct method to detect the expression levels of BRMS1 mRNA and MACC1 mRNA in colorectal carcinoma and adjacent normal colorectal tissues, and investigate the association of their expression with clinical parameters.The experimental data were analyzed by SPSS 17.0 software,the relationship between the expression of BRMS1 and MACC1 and clinicopathological parameters of colorectal carcinoma were analyzed by two independent sample t test. The correlation between BRMS1 and MACC1 were analyzed by Pearson correlation analysis.The statistical results with P <0.05 (bilateral) was considered to be statistically significant.Results:The expression of BRMS1 mRNA and MACC1 mRNA in colorectal carcinoma tissues and adjacent normal colorectal tissues was significant difference.1. The expression of BRMS1 mRNA in colorectal carcinoma was significantly lower than that in adjacent normal tissues.The expression levels of BRMS1 was correlated with the regional lymph node metastasis,depth of invasion,TNM stage,differentiation degree,distant metastasis and Dukes stage (P< 0.05).They were not correlated with the patient’s age, gender, tumor location and tumor size (P>0.05)2. The expression of MACC1 mRNA in colorectal carcinoma was significantly higher than that in adjacent normal tissues.The expression levels of MACC1 was correlated with the regional lymph node metastasis,depth of invasion,TNM stage,differentiation degree,distant metastasis, Dukes stage and tumor size (P<0.05).They were not correlated with the patient’s age, gender, and tumor location (P>0.05)3. The expression of BRMS1 mRNA was negatively correlated with the expression of MACC1 mRNA in colorectal carcinoma tissues(r=-0.430, P<0.05).Conclusion:The expression of BRMS1 mRNA and MACC1 mRNA in colorectal carcinoma tissues was correlated with the differentiation degree,tumor metastasis and tumor stage,their abnormal expression may be involved in the occurrence, progression and metastasis of colorectal carcinoma.There is a negative correlation between the expression of BRMS1 and MACC1. Combined detection of BRMS1 and MACC1 can be used as important index to judgment biological behavior of colorectal carcinoma, and that will contribute to the early diagnosis and prognosis evaluation of patients with colorectal carcinoma.