| Lung cancer is one of the most common malignant tumor whose patients representing the first of all malignant tumor. As people’s attention and research on lung cancer gradually deepening, the prevention and treatment of lung cancer has made some progress. However, the incidence and morality of lung cancer are still increasing year by year. Non-small cell lung cancer patients always diagnosed in late stage with metastasis who lost the chance of operation for it happened with no sign. Therefore, chemotherapy is the main treatment of non-small cell lung cancer. Micro RNAs is a kind of small non-coding RNA molecule which can regulate gene expression. Circulating micro RNAs is a kind of novel small molecule which present stable and objective in serum. Numerous studies have demonstrated that circulating micro RNAs could be non-invasive markers who have extensive application for diagnosis, treatment and prognosis of non-small lung cancer. And it could be a new direction to looking for effective markers for diagnosis and evaluation of chemotherapy of non-small cell lung cancer.Purpose:To explore the correlation between serum expression of mi R-19 b, mi R-125 b,mi R-34 a, mi R-200 b with the development and clinical pathological features of non-smallcell lung cancer. To investigate the function of chemotherapy evaluation and changes of expression levels during treatment of these four micro RNAs, which can provides new research direction of diagnosis and the efficacy of chemotherapy evaluation for non-small cell lung cancer.Methods:Detected the changes of serum expression levels before and after chemotherapy of mi R-19 b, mi R-125 b, mi R-34 a, mi R-200 b with real-time fluorescence quantitative PCR,and then compared with 43 cases of healthy persons. Meanwhile, the patients were divided into two groups including effective group(complete response + partial response),ineffective group(disease stable and disease progression) based on the efficacy after six cycles of chemotherapy. Compared the changes of expression levels of mi R-19 b,mi R-125 b, mi R-34 a, mi R-200 b between groups and the content difference after the second and six chemotherapy.Results:1. Compared with the healthy control group,the serum expression levels of the four micro RNAs in patients of lung cancer have a statistically significant(P< 0.05). According to the relative expression of mi R-19 b, mi R-125 b, mi R-34 a, mi R-200 b in health and lung cancer patients, we made ROC curves,and the area under the curve were 0.8734, 0.826,0.7296, 0.8702 respectively.2. The expression levels of these four micro RNAs were not correlated with age, sex,smoking and pathological type. However, mi R-19 b, mi R-125 b, mi R-200 b were correlated with lymph node metastasis(P were 0.041, 0.001, 0.009 respectively), while mi R-34 a was not associated with lymph node metastasis which P was 0.455.3. In the 43 cases of non-small cell lung cancer patients, 11 patients with stage IIIA, 7patients with stage IIIB, 25 patients with stage IV. mi R-19b: compared patients in stage IIIA with patients in stage IIIB, q of mi R-19 b was 3.727, P<0.05; Compared patients in stage IIIA with patients in stage IV, q was 6.573, P<0.05; When compared patients in stage IIIB with patients in stage IV, q was 1.348, P>0.05. mi R-125b: compared patients in stage IIIA with patients in stage IIIB, q of mi R-125 b was 0.195, P > 0.05; Comparedpatients in stage IIIA with patients in stage IV, q was 11.44, P<0.05; When compared patients in stage IIIB with patients in stage IV, q was 9.463, P< 0.05. mi R-34a:no matter the comparison between patients in stage IIIA and stage IIIB, stage IIIA and stage IV, or stage IIIB and stage IV, P>0.05. mi R-200b: compared patients in stage IIIA with patients in stage IIIB, q was 14.2, P<0.05; Compared patients in stage IIIA with patients in stage IV, q was 9.113, P<0.05; When compared patients in stage IIIA with patients in stage IIIB, q was 2.564, P>0.05.3. In the effective group(CR+PR), the serum expression levels of mi R-19 b, mi R-125 b were gradually decreased, the micro RNAs content of the last chemotherapy was significantly lower than the second chemotherapy(P<0.05). When in the ineffective group(SD+PD), the result was just the opposite. The expression levels of mi R-19 b and mi R-125 b were increasing which had significant difference between different stages of chemotherapy(P<0.05). There was no difference between effective group and ineffective group of mi R-34 a after chemotherapy(P>0.05). The expression of mi R-200 b was decreased both in effective group and ineffective group after chemotherapy, and there was significant difference between the second and sixth chemotherapy.Conclusions:1. The serum expression of mi R-19 b, mi R-125 b, mi R-200 b were increased significantly in patients with non-small cell lung cancer, while the serum expression of mi R-34 a was decreased. And they all had significant difference when compared with the healthy control group. It indicated that mi R-19 b, mi R-125 b, mi R-34 a, mi R-200 b could be potential specific serum markers for diagnosis of lung cancer.2. The expression of mi R-19 b, mi R-125 b, mi R-34 a, mi R-200 b were not correlated with sex, age, smoking, histological type. Howere, mi R-19 b, mi R-125 b, mi R-200 b were correlated with lymph node metastasis, the expression were all increased which may indicate lymph node metastasis.3. mi R-19 b and mi R-125 b could be an indication for chemotherapy efficacy. We need to change the current chemotherapy plan if the expression of these two micro RNAs continued to rise, because it was a sign that the chemotherapy was not valid. It indicatedthat the chemotherapy was effective when the expression of these two micro RNAs continued to decline. |
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