Knockdown Of PES1 Inhibits Migration And Invasion Of MDA-MB-231 Breast Cancer Cell

Background: In the past few decades, the incidence of cancer in China has been rising greatly, especially of lung cancer, breast cancer, prostate cancer etc. Breast cancer is one of the major threats to women. Although diagnosis and treatment of breast cancer has made great improvement with the progress of science and technology, the mortality is still high. Many factors can affect the therapeutic effect of cancer patients, especially metastatic and invasive ability of cancer cells. Through the lymphatic vessels and/or blood vessels, cancer cells can be transferred to distant organs, such as brain, lung, liver etc and cause death.Epithelial mesenchymal transition(EMT) plays an important role in migration and invasion of tumor cells, it is a process that epithelial cells transformed to cells with mesenchymal phenotype through specific program. EMT plays an important role in embryonic development, chronic inflammation, tissue remodeling, cancer metastasis,which is characterized in the expression of cell adhesion molecules, reduced cytokeratin cytoskeleton vimentin(Vimentin) into the cytoskeleton and morphology character of mesenchymal cells etc. In the process of EMT, epithelial cells lose cell polarity and connection to basal membrane, gain a higher migration and invasion ability of stromal cells characters.The PES1 gene is found when studying embryo developmental defects of Zebrafish induced by retrovirus. It plays an important role in ribosome biogenesis, cell cycle and cell proliferation. PES1 has high expression in breast cancer cells and plays a role by regulating expression of ER alpha and ER beta of steroid hormone signal transduction pathways, it can enhance the stability of ER alpha while reduced expression of ER beta.In ER positive breast cancer, PES1 regulates balance of estrogen receptor alpha and estrogen receptor beta to influence the development of breast cancer. In ER negative breast cancer cells, PES1 works as an oncogene to promote cell proliferation. So far, it is unclear whether PES1 affects the migration and invasion of tumor cells.Objective: To construct MDA-MB-231 breast cancer cell line stably expressing PES1 sh RNA and to study its effect on MDA-MB-231 cell migration and invasion.Methods:1. PES1 sh RNA primers were designed and systhesed and constructed into p SHI1-H1-Puro plasmid vector. Constructed plasmid was packaged with lentivirus and titer was quantified. MDA-MB-231 stable cell line was established by transfection,expression of PES1 gene was quantified by RT-q PCR for m RNA and Western blot for protein.2. Cell migration ability was measured by cell scratch method.3. Invasion ability was measured by Transwell experiment.Results and conclusion: Lentiviral vector for knockdown PES1 gene was established and MDA-MB-231 cell lines stably express PES1 sh RNA were constructed.Knockdown of PES1 expression inhibits migration and invasion ability of MDA-MB-231 cells.