Characteristics Of Gene Polymorphism And Atherosclerotic Ischemic Stroke Vascular Distribution

Objective:focus on the clinical phenotype of ischemic stroke analysis, in order to reveal the genetic background of cerebrovascular disease, screening susceptible patients with hereditary cerebrovascular disease, targeted to take warning and individualized therapy.Methods: blood samples were collected in 100 patients with ischemic stroke, anterior circulation vasculardistribution of vascular distribution area of infarction with 50 cases in each area and after infarction. Blood samples were extracted by means of detection of DNA, APOE, ALOX5 AP of high through put technology, MMP-2, MMP-9,ATG5, ATG7, ATG8 gene was associated with the SNP locus of the strong screening assay.Results:(1) before and after cyclic group patients circular group atherosclerotic ischemic stroke in the ApoE gene ofrs769450 base type is TT, the incidence of equal proportion, no significant difference(P>0.05)(2) before and after cyclic group patients circular group atherosclerotic ischemic stroke in the AL0X5 AP gene of rs4769055, rs9579645, rs4075132, rs4503649, rs10162089, rs12431114, rs4254165, r s3935644 and rs4769060 loci results are different. Among them:â‘ The rs4769055 locus of wild type CC, rs4075132 loci of wild type TT, rs10162089 loci of wild type AA, rs4769060 site mutant GG, rs4503649 loci of wild type CC and mutant AA, rs4254165 loci of wild type AA and mutant GG and rs3935644 loci of wild type AA and mutant GG is susceptible to posterior circulation infarction, the difference has statistical significance(P<0.05).â‘¡The rs4769055 locus, the rs10162089 locus of mutant AA mutant GG and rs4503649, rs4254165, rs3935644 and rs4075132 heterozygous sites prone to anterior circulation infarction, the difference was statistically significant(P<0.05).â‘¢The heterozygotes at the rs4769055 locus and rs10162089 locus heterozygotes circular group incidence ratio in the front, rear, no significantdifference(P>0.05).(3) before and after cyclic group patients circular group atherosclerotic ischemic stroke respectively in the genes MMP-2 and MMP-9 in different SNP locus results are different. Among them:â‘ Rs865094 MMP-2 gene in heterozygous sites, rs17301608 sites, rs11639960 sites heterozygous mutant GG and rs243835 heterozygous sites prone to anterior circulation infarction, the difference was statistically significant(P<0.05); rs17301608 loci of wild type CC, rs11639960 loci of wild type AA and rs243835 loci of wild type TT prone to posterior circulation infarction, there is statistical significant differences(P<0.05); rs11639960 heterozygous sites circular group incidence ratio in the front, rear, no significant difference(P>0.05).â‘¡Not related to the distribution of MMP-9 in vascular gene rs2274755, rs17576 andrs3918254 loci and theiratheroscl erotic ischemic stroke, no significant difference(P>0.05).(4) before and after cyclic group patients circular group atherosclerotic ischemic stroke respectively in theautophagy related gene Atg5, 7, 8 genes of different related SNP locus results are different. Among them:â‘ The loci of rs633724 and rs2787540 of Atg5 gene in heterozygous sites prone to posterior circulation infarction,the difference was statistically significant(P<0.05).â‘¡Rs921226 II Atg7 gene in heterozygous sites, rs7625184 sites, rs3816380 heterozygote heterozygous sites,rs11128547 sites, rs6791877 heterozygote heterozygous sites, rs2454511 sites, rs4684073 heterozygoteheterozygous sites, rs6442260 sites, rs346078 sites heterozygous mutant GG, the mutation of rs2594992 type AAand rs4684787 loci of wild type CC easily happened before circulation infarction, the difference was statistically significant(P<0.05); rs346078 loci of wild type CC, rs2594992 loci of wild type CC, rs3816380 loci of wild type AA,rs6791877 loci of wild type AA, rs2454511 loci of wild type CC, rs4684073 loci of wild type AA and rs4684787 mutation type TT is susceptible to posterior circulation infarction, the difference was statistically significant(P<0.05); rs346078 heterozygous sites, rs2594992 sites and rs4684787 heterozygous sites before, posterior circulation group the incidence rate, no significant difference(P>0.05).â‘¢Not related to vascular distribution of Atg8 gene in SNP loci associated with atherosclerotic ischemic stroke, no significant difference(P>0.05).Conclusion: ALOX5 AP, MMP,-2, Atg5 7 gene may cause SNP gene polymorphism sites related by regulation,vascular distribution and dynamic effect of atherosclerotic ischemic stroke.