Peripheral Blood CD8~+T Cells And Ig A Level, Variation And Their Significance In Adult Patients With Epilepsy

Objective: To observe the effect of adult epilepsy patients peripheral blood T lymphocyte subsets, immunoglobulin, complement, glutamic acid decarboxylase antibody(GADA) and anti nuclear antibody(ANA) level. Analysis cellular and humoral immunity state in the patients with epilepsy, and to explore the role of immune abnormalities in the pathogenesis of adult epilepsy, in order to provide a new way for the prevention and treatment of epilepsy.Methods: 52 cases of adult epilepsy patients were selected as case group, and another 25 helthy subjects were selected as the normal control group, both of them meet the met the standard of included and excluded protocols. All groups except excluded significant differences age, gender. The patients according to etiology for cryptogenic epilepsy group(28 cases) and secondary epilepsy group(24 cases); by taking into epilepsy no medicine group(20 cases) and regular medication group(32 cases). Were recorded age, sex, age of onset, type of epilepsy, medication history, past medical history, personal history, family neural and cognitive function, electroencephalogram and cranial MRI data. All subjects were collected in the morning fasting 2ml without anticoagulation and 2ml sodium citrate anticoagulation elbow venous blood using flow cytometry(FCM) percentage to check all the research object CD3+, CD4+, CD8+ T cells, nephelometry and serum Ig G, Ig A, Ig M, C3, C4 level by means of immune scattering, application Western-blot and ELISA detection of peripheral blood GADA and ANA levels. Application SPSS20.0 statistical software for data processing and statistical analysis, significant test level of bilateral inspection P<0.05.Results:1. The comparison of T cell subpopulations in Adult cryptogenic epilepsy group, secondary epilepsy group and normal control group,we found that: CD8+Tcells increased and CD4+/CD8+ ratio decreased in cryptogenic epilepsy group, there were significant differences compared with normal control group(P < 0.05); CD8+Tcells increased in secondary epilepsy group, and statistical significance were found compared with the normal control group(P < 0.05); No significant difference were found between the two groups with epilepsy(P > 0.05).2. The comparison of T cell subpopulations in Adult cryptogenic epilepsy group, secondary epilepsy without taking medicine group and normal control group,we found that: CD8+Tcells increased in cryptogenic epilepsy without taking medicine group, there were significant differences compared with secondary epilepsy without taking medicine group and normal control group(P < 0.05); CD4+/CD8+ ratio decreased in cryptogenic and secondary epilepsy without taking medicine group compared with the control group had significant difference(P < 0.05).3. The comparison between the different causes of epilepsy and normal control group Ig G, Ig A, Ig M, C3, C4 levels,we found that: Ig A level in cryptogenic epilepsy group was decreased, there were significant differences compared with normal control group(P < 0.05). C3 level decreased in Secondary epilepsy group, here were significant differences compared with normal control group(P < 0.05), C4 increased compared with cryptogenic epilepsy group had significant difference(P < 0.05).4. The comparison between cryptogenic epilepsy without medication, medication group and normal control group, Ig G, Ig A, Ig M, C3, C4 levels.we found that: Ig A level decreased in cryptogenic epilepsy no medicine group, there were significant differences compared with normal control group(P < 0.05), C3 decreased in cryptogenic epilepsy medication group, there were significant differences compared with medication group group(P < 0.05).5. The comparison between secondary epilepsy without medication, medication group and normal control group, Ig G, Ig A,Ig M, C3, C4 levels group. we found that: C3decreased in secondary epilepsy medication group, there were significant differences compared with the control group(P < 0.05).6. The comparison with the positive rate of serum GADA,ANA in cryptogenic epilepsy group and the secondary epilepsy group, no significant difference were found between the two groups with epilepsy.The positive rate of ANA increased in epilepsy group, compared with the control group(P < 0.05), of which the positive rate of group ANA was higher than that of no medicine group(P < 0.05).Conclusion:1. Cellular and humoral immune function disorder existed in adult patients with epilepsy, and the disorder existed before any anti-epileptic drug was taken. However, not all patients with epilepsy had immune dysfunction.2. Immune dysfunction was more obviously observed in patient with cryptogenic epilepsy than in patient with secondary epilepsy.Cellular immune disorder was more prominent.3. Our study found that abnormality of CD8+Tcells was the most common form of cellular immune dysfunction in patients with epilepsy.