Effects Of Ginsenoside RG3 On The Proliferation And Differentiation Of 3T3-L1 Adipocyte And The Related Mechanism

Object:Effects of Rg3 on proliferation and differentiation of 3T3-L1 cells, and to explore its mechanism.Method:Experiments in vitro:Rg3 on different concentration treatment, the control group has no Rg3. Detection method of ATP effect on 3T3-L1 cells in vitro fat added ginsenoside Rg3;detection of ROS levels; The expressions of RT-PCR and Western bolt detection of gene and protein.Experiments in vivo:1.25 ICR mice were divided into normal diet group and high fat diet group. The high fat diet group was divided into the positive control group and the intervention group, and the intervention group was administered with Rg3 for 20 days.2. Detection of body weight, epididymis fat and liver fat content.Result:Reults in vitro:l.Rg3-0.1 compared to the positive control group, can promote 3T3-L1 apoptosis increased, has difference significance (P<0.05),show that Rg3 has effects of promoting fat cell apoptosis in a certain range of concentration.2.Rg3-0.01 group and Rg3-0.1 group compared with the positive control group, ATP content increased obesity 3T3-L1 (P<0.05).3. Rg3-0.01 group ROS level although the decline, but do not has significant, The ROS level of Rg3-0.1 has significant reduce (P<0.05).4.RT-PCR results:①PAR-y mRNA:Rg3 in a certain range of concentration reduced fat in the process of expression of key transcription factor PPAR-yand C/EBP protein level, protein expression about PPAR-y was decline(P<0.01),PPAR-γ expression of Rg3-0.1 group was less than the positive control group (P< 0.001).②C/EBPa mRNA Expression of C/EBP alpha Rg3-0.1 intervention group was less than positive control group (P＜0.001).③SREBP1 mRNA:The Rg3 intervention group and Rg3-0.1 group were compared with the positive control group, the expression of mRNA was significantly inhibited, Rg3-0.01 expression was significantly different(P<0.05; P<0.01).④ FAS mRNA:The expression of FAS mRNA was significantly different between the Rg3-0.1 group and the positive control group (P<0.05).5. Weston bolt results:Rg3-0.01 group compared with the positive control group significantly different (P< 0.01). PPAR-y protein expression of Rg3-0.01 group lower than the positive control group (P< 0.1, P< 0.001). C/EBPa protein expression of the Rg3-0.1 group lower than the positive control group (P< 0.001).Reults in vivo:1.The weight of mice treated with high fat diet after Rg3 treatment was significantly decreased (P<0.001)2.Rg3-1 group, Rg3-3 group were compared with the positive control group, liver fat weight significantly decreased (P< 0.001). The weight epididymis fat of Rg3-1 group and Rg3-3 group were significantly lighter than the positive control group (P<0.05).Conclusion: Rg3 can improved 3T3-L1 cell viability, and inhibit 3T3-L1 adipocyte differentiation, can promote fat cell apoptosis, depressed cell proliferation, also deduced the mRNA and the protein levels,can reduce the weight and body fat weight of high fat diet mice,ginsenoside weight,regulate amylaceum and the process of fattiness metabolism balance function.