High Song Pingwei Inhibition Of Mouse Fat Cell Differentiation And Prevention Of Obesity Mechanism Of Experimental Research

Objective: To study the effect, mechanisms of Zhaqupingweisan solution on differentiation in primary mouse preadipocytes in vitro and prevention from to be obese , reducing weight in nutritional obesity mice in vivo.Methods: (1) We observed the morphological changes and post Zhaqupingweisan solution added into cultured primary mouse preadipocytes in vitro. We detected the quantity of intracytoplasmic lipids by oil red 0 staining and the quantity of GPDH in the cytoplasm of differentiated adipocytes. And the expression of C/EBPα , PPARγ, ADD1/SREBP1 and Pref-1 mRNA relating to differentiation of adipocytes was detected by RT-PCR. (2) During the process of in vivo, we observed body weight, obese index, fat weight, weight ratio, adipocyte size and blood lipid.Results: (1) During the process of in vitro, the lipid droplet was less and smaller treated with Zhaqupingweisan solution as compared with the untreated control cells. The quantity of intracytoplasmic lipids and GPDH was less than the untreated control cells. RT-PCR showed that Zhaqupingweisan solution reduced the expression, of C/EBPα, PPARγ, ADD1/SREBP1 mRNA and increased the expression of Pref-1 mRNA. The groups of 4mg/ml and 6 mg/ml showed noticeable effects. (2) During the process of in vivo, body weight, obese index, fat weight, weight ratio, adipocyte size and blood lipid in the three Zhaqupingweisan solution groups were...