| Objective: Useing the method of HPLC determ the concentration of five main alkaloids (berberine, palmatine, jatrorrhizine,coptisine,table berberine, jatrorrhizine) of coptidis in rats’ plasma, and compare the difference of pharmacokinetic parameters of the five kinds of alkaloids mentioned above between the groups of rats which orally administered coptidis or coptidis-ginger extracts. Methods:Sixteen Wistar rats(250±20g) of either sex were used and fed with standard food for two weeks, and then divided into two groups(n=8):the high-dose group and low-dose group. After orally administration (0.86ml/100g) of Coptidis’extrcats blood samples were collected on the time point of0.0833h,0.1667h,0.25h,0.5h,0.75,1h,1.5h,2h,3h,4h,6h,8h,12h,24h. Keep these rat for seven days, according to the same volume administered to rats berberine groups-ginger extract water, and on the same point in time than blood samples. When using high-performance liquid chromatography five alkaloids plasma concentration of rats in each group at each time point on, draw five kinds of alkaloid drugs curve, and fit with DAS1.0(Drug and Statistics for Windows1.0) calculate the pharmacokinetic parameters of the alkaloids, calculated using the Excel spreadsheet after the two were compared before and after the administration of the pharmacokinetic parameters. Results: Specificity proved blank plasma absorption peaks for each alkaloid no effect, according to the sample concentration and peak area measured at seven different concentrations of points, Coptidis’regression equation y=2.9e+5x+1975.2, r2=0.9993, palmatine regression equation y=3.8e+5x-3160.5, r2=0.9991, coptisine regression equation y=3.2e+5x-1791.4, r2=0.9991, Table berberine regression equation y=4.2e+5x-3412.1, r2=0.9992, jatrorrhizine regression equation y=3.3e+5x-2678.6, r2=0.9992. Described in this linear range, berberine, palmatine, berberine, berberine table, a good linear relationship jatrorrhizine, and precision, reproducibility and RSD value recovery test results were available the acceptable range, and the detection and-20℃refrigerated24h30days refrigerated samples4℃, sample concentrations were measured over a fresh sample concentration of95%, indicating good sample stability, there is no decomposition phenomenon. Pharmacokinetic experimental data show that compared with berberine group, berberine-Ginger high dose group and low dose group t1/2z, MRTO-t, MRT0-∞were significantly lower (p<0.01or p <0.05), In addition to the outside and berberine berberine-Ginger and high dose groups each alkaloid AUCO-t, AUC0-∞compared with berberine high dose group was significantly lower (p<0.01or p<0.05), and we found that berberine-Ginger low dose berberine group AUC0-and coptisine table berberine, jatrorrhizine of AUC0-t, AUC0-∞were higher than berberine-Ginger high-dose group (p<0.01). Conclusion:In this study, the use of high performance liquid chromatography determination of the plasma concentration in rat plasma berberine, palmatine, berberine, berberine table, jatrorrhizine five alkaloids. By examining the specificity of the method, linearity, precision, recovery and stability, the experimental results prove that the good reproducibility and high reliability; compared with berberine group, berberine ginger group t1/2z, MRTO-t, MRTO-∞were significantly reduced, and berberine-Ginger and high dose groups each alkaloid AUCO-t, AUCO-∞berberine significantly lower than the high-dose group; ginger can accelerate rats each alkaloid The elimination rate; ginger and in some cases also can promote the absorption of berberine alkaloids. |
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