The Extraction And Separation Of Coptisine And Its Antiobesity Effect And Mechanism Research

Obesity is a syndrome which formed by interaction of various factors, the cause of its formation is complicated. Obesity can cause the body structure, the adaptability changes of the nervous system and internal environment, which lead to the diseases that be associated with metabolic disorders such as high blood pressure, diabetes, cardiovascular disease. In addition, obesity can lead to stroke, osteoarthritis, sleep apnea, certain types of chronic diseases such as inflammation and cancer. Obesity is not only a severe challenge to human health, it is also the world’s attention of major public health problem. The cause of obesity is complicated, can be divided into simple obesity that have no obvious reason of and secondary to obesity that be secondary to the diseases such as malnutrition, endocrine, hypothalamus-pituitary tumor. Most of the occurrence of obesity is simple obesity due to the body nutrition excess energy intake than energy expenditure. Weight loss is to point to human body in health state, subtractive redundant adipose, reach the purpose of reduce weight and body fat, so as to control the body’s body fat at a suitable level. At present the treatment of obesity ways mainly control the diet, increase the movement, surgical treatment, drug therapy, etc. Most of the obesity patients using single method reducing weight, weight easy rebound after stop losing weight. Nowadays people lose weight often give priority to in order to control diet and increased physical activity, medication is complementary. Obesity drugs have lipase inhibitors and glycosidase inhibitor etc. Obesity drug therapy is a controversial issue, with the understanding of the pathogenesis of obesity, antiobesity drugs are now gradually become obesity treatment means for a better. But the current clinical application of the medicine that reducing weight more or less have some adverse reactions, therefore, to find a safe and effective antiobesity drug become a research hotspot.Rhizoma coptidis to ranunculaceae plants coptis chinensis (Coptischinensis Franeh), triangular leaf coptis chinensis (CoptisdeltoideaC. Y.c hengetHsiao) and a cloud (CoptisteetaWall), dried roots has clear heat, dry wet detoxifying of purging fire. Studies showed that rhizoma coptidis has the effect such as anti-cancer, antioxidant, antimicrobial, anti-inflammatory, antiviral, antipyretic, fall blood sugar, fall hematic fat, fall blood pressure, dilate blood vessels, analgesia, platelet aggregation resistance, strong heart, anti-arrhythmic and cholagogic pharmacological. Its main active ingredients of alkaloids including berberine, coptisine, jatrorrhizine, epiberberine, palmatine. A large number of studies have shown that rhizoma coptidis have fall hematic fat, adjust the function of cholesterol metabolism in the body. The main alkaloid berberine in rhizoma coptidis, repeatedly was reported to have good lipid-lowering effect. And a large number of clinical trials showed that berberine low toxicity and less side effects, but because of the molecular structure of the berberine, make its low solubility in water, oral absorption is not good, occasionally there are some side effects such as mild constipation. Coptisine, of quaternary protoberberine alkaloid (QPA), which is one of the main active ingredients in rhizoma coptidis, which have the similar physiological activity with berberine. In this study, the obesity induced by high fat feedstuff golden mice as model, the antiobeisity effect and mechanism of coptisine on golden hamsters were evaluated.The main contents and results of this study:(1) Coptisine with a purity of 98% was successfully isolated and purified from RC.(2) The experiments have found that high fat cholesterol diet can induce weight, TC, TG, LDL-c levels increase and low HDL-c.4 weeks after the treatment, hydrastis showed good improve the weight and the activity of lipid-lowering effect. Especially hydrastis on 70.05 mg/kg dose, improve body weight change, Lee’s index and significantly reduce TC respectively, TG and LDL-c 28.9%,26.6% and 17.14%, 15.85% higher HDL-c (P<0.01 or P<0.05). In addition, hydrastis increased significantly in feces, free fatty acids, bile acids excretion.(3) The protein level and mRNA expression of genes involved inlipid metabolism were analyzed by ELISA and qRT-PCR. The results have revealed that compared to HFHC group, coptisine could reduced the leptin levels and increased apoB levels in Syrian golden hamsters. Coptisine could significantly reduce the FAS mRNA and protein expression to reduce the synthesis of fatty acids and triglycerides; resulting the decrease of the lipid synthesis in the liver; coptisine can also increased the protein and mRNA expression of LDLR.In conclusion, coptisine was a potential safe anti-obesity agent from rhizoma coptidis which could improve promote lipid discharge, the utilization, transport and excretion of lipid by regulating the protein and mRNA expression of FAS, LDLR.