Screening Of Genes Related To Colon Cancer By Gene Expression Profiling Chip

Objective:To explore the key genes and pathways in the tumorigenesis of colon cancer, make a better understanding on the genetic mechanism, and lay the theoretical foundation to the early diagnosis, treatment and prognosis, we analyzed the genes in the tumorigenesis and development of colon cancer, and screened the mutant genes by using Agilent human whole genomic oligonucleotide microarrays.Methods:RNA of colon cancer tissue and the paired normal colon tissue was extracted and then cRNA was synthesized. The cRNA was labeled, hybridized and scan by using Agilent human whole genomic oligonucleotide microarrays to form the expression profiling data. Differentially expressed genes related with colon cancer were screened, and analyzed further by GO term analysis, KEGG Pathway analysis and protein interaction analysis.Results:13986differentially expressed genes were found by the gene expression profiling chip, among which2807genes were up-regulate genes and1179were down-regulate.2.Wnt signaling pathway, PI3K-Akt signaling pathwat, Jak-STAT signaling pathway, ErbB signaling pathway, MAPK signaling pathway, Cell Cycle, TGF-β signaling pathway and apoptosis were the main signaling pathways found that related to the tumorigenesis of colon cancer.24differential genes were found in these pathways.Conclusion:5differential genes(ACVR1B, AKT3, CYCS, MAPK9, PIK3CB) were found in our research for the first time to be relevant to the tumorigenesis of colon cancer, and might be new therapeutic targets in colon cancer. Further analysis of these key genes would lay a theoretical foundation to the early diagnosis and treatment of colon.