| Research background and objective:Tendon injuries of hand injuries accounted for aboutone-third of open injuries. Adhesion during healing of tendoninjury would seriously affect the recovery of hand function.Studies have shown that tendon adhesion is mainly due toinvolvement of inflammation in tendon exogenous healing, andcertain medications such as curcumin can inhibit theinflammation, so we studied the inhibition of curcumin on localinflammation so as to avoid adhesion and the differencesbetween curcumin and nano-curcumin in preventing tendonadhesion.Materials and methods:1. Materials:72healthy male Wistar rats (body weight:350-450g) were selected as the experimental models.2. Methods: The rats were raised routinely in animal labfor1week and when they were stable the experiment was carriedout.72rats were randomly divided into3groups for Achillestendon reflex test: the blank control group (group A); curcumin group (group B); and combination group where nano-curcumin wasapplied with laser irradiation (group C).3groups were givenphysiological saline, curcumin and nano-curcumin,respectively. And rats were given tendon test at different timepoints during postoperative healing (1w,2w,4w and8w) in grossmorphology, tissue morphology, biomechanics andimmunohistochemistry. Adhesion degrees of rat Achilles tendonbetween the experiment group and the control group and betweenthe two experimental groups were analyzed.Results1. Gross finding:Rat Achilles tendon of each group showed mild adhesion on1st week after operation, and the differences were not obvious;while after2rd week, adhesion was aggravated; and thedifferences between group A and group B were not obvious; butadhesion degree of group C was significantly better than theother groups;4weeks after operation, their adhesion becameeven serious; adhesion degree of group A was more serious thangroup B, but the differences between the two groups were smaller;and adhesion degree of group C was obviously better than theother two groups; on8thweek after operation, rat Achillestendon adhesion was better than before; rats Achilles tendon adhesion degree was gradually reduced from group A, B to C;differences between group A and group B was smaller than thatbetween group B and group C.2. Tissue morphology:Different degrees of hyperemia edema and inflammatoryinfiltration can be seen in the fracture end of rat tendon at1week after operation; anastomotic inflammation in group A andgroup B was level3, but that of group C should be level2.2weeks after operation, hyperemia edema and inflammatoryinfiltration were better.4weeks after operation,inflammation was gradually subsided, fibroblast proliferatedand moved to the fracture end, and disorganized collagen fiberscan be seen in the fracture end; compared to the other two groups,collagen fibers of group C were better arranged;8weeks afteroperation, fibroblasts at fracture end of rat Achilles tendonin each group were decreased, and collagen fibers were moreorderly arranged than before; and compared to the other groups,group C was more orderly arranged.1.Biomechanical test:With regard to tendon movements on postoperative4weeksand8weeks in each group, group A and group B were reduced whilethat of group C was increased; for comparison on maximum tensile resistance on postoperative4weeks and8weeks, there were noobvious differences between groups (P>0.05). Comparison oneach time point during postoperative4weeks and8weeks, tendonmovement of group C was significantly larger than the other twogroups; and maximum tensile resistance was gradually reducedfrom group A, via group B, to group C.4. ImmunohistochemistryRats of group C had higher FGF-2antibodies, FGFR-1antibodies, and β-catenin antibodies than the other two groups,while EPCAM antibodies were significantly lower than the othertwo groups.Conclusion1. Nano curcumin has certain inhibitory effect ofinflammation.2. Nano curcumin has the effect to prevent tendon adhesion.3.The nano curcumin can promote tendon endogenous healing. |
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