Screen For Lung Cancer With Serum Surface Enhanced Raman Spectra Technology

Surface-enhanced Raman Spectroscopy (SERS) technique is available for detecting trace materials, which is marked with Raman characteristic spectrum. It has advantages in SERS sensitivity, resolution, stability, detection time, and the amount of samples which are needed, etc. SERS technology has been widely used in various scientific analysis fields, such as material, environment, chemistry, biological, archaeology, etc.This paper firstly expounds the principle of Raman spectrum and the theoretical explanation, and focuses on the introduction of the characteristics and the enhancement mechanism of surface enhanced Raman scattering effect. Then importantly introduce the preparation method of Raman active base of silver colloid after filter millipore and study it’s SERS enhancement activity, and when detect serum Raman spectroscopy, we find that the Raman signal strength is stronger and the signal noise ratio (SNR) is better. This shows that this active base of silver colloid after filter millipore can be used in the Raman detecting of trace substances. Last but not the least, on the basic of Raman active substrate of silver colloid after filter millipore, we collect the serum surface-enhanced Raman spectroscopy (SERS) signal of normal people (the number is41) and patients with lung cancer (the number is43) by using this active substrate. The original data are processed with method of baseline calibration and normalized with725cm-1spectral peak. Then the normalized relative intensity of Raman spectra are analysed with method of principal component analysis (PCA), and the purpose is to explore a new method of screening for lung cancer. On the basis of clinical pathologic diagnosis, the result of this screening is that only two lung cancer patients of84samples have not been properly selected (misjudgment rate is4.7%) and the misjudgment rate about normal people is0%, and the accuracy is as high as95%for the screening of lung cancer patients. The primary results show that the method of serum-SERS screening for the patients with lung cancer is successful, and it has the further practical application prospect.