Epidemic Investigation Of PMQR Factor Among Quinolone-Resistance Escherichia Coli From Different Animal Sources In Xinjiang

To investigate resistance to clinical commonly used antimicrobial from different animal source of E. coliand the drug resistance gene within them in xinjiang, Minimal inhibitory concentrations (MIC) method wereapplied for determine the broth micro-dilution of the antimicrobial drugs to these isolates from anal swab of pigsource, sheep source and cattle source. Results showed that:(1)454E. coli isolates were confirmed from543pigfecals samples (83.6%), the resistance rates of enrofloxacin, norfloxacin, ciprofloxacin and florfenicol were42%,42%,20%, and54%; multiple resistance of2or more accounted for37.5%.(2)638E. coli isolates wereconfirmed from780ovine fecals samples (81.8%), the resistance rates of ciprofloxacin, enrofloxacin, norfloxacin,and florfenicol were15.67%,14.58%,7.84%and3.1%,0-2resistant strains accounted for87.6%.(3)89E. coliisolates were confirmed from90bovine fecals samples (98.9%), the resistance rates of enrofloxacin,ciprofloxacin and norfloxacin were3.6%～6.7%,6.7%, and6.7%, more than2and2resistant strains just2strains, accounting for2.3%.Using polymerase chain reaction (PCR) to detect the PMQR factor and β-lactamase16s rRNA genes andmethylation enzyme gene of quinolone resistant type of E. coli in pig source (79strains), cattle source (8strains)and sheep source (96strains),DNA sequencing on purpose stripe. Results showed that:(1) that the porcine E.coli carrying qnrS (5/79,6.33%), oqxA (35/79,44.30%), oqxB (40/79,50.60%), aac(6’)-Ib-cr (4/79,5.06%),blaTEM(79/79,100%), rmtB (3/79,3.80%),2isolates were found to possess the qnrS, oqxA, oqxB, aac(6’)-Ib-cr,rmtB and blaTEMgenes in the same time,2isolates were found to possess the qnrS, oqxA, oqxB, rmtB and blaTEMgenes in the same time;(2) cattle source carrying qnrS (1/8,12.50%), oqxA (1/8,12.50%), oqxB (1/8,12.50%),aac(6’)-Ib-cr (1/8,12.50%), qepA (1/8,12.50%), blaTEM(8/8,100%), blaSHV(1/8，12.50%), only1isolate wasfound to possess the qepA, oqxA, oqxB, blaTEMand blaSHVgenes in the same time;(3) Sheep source carryingqnrS (6/96,6.25%), oqxA (32/96,33.3%), oqxB (37/96,38.5%), aac(6’)-Ib-cr (22/96,22.91%), blaTEM(96/96,100%), blaCTX-M(2/96,2.08%), rmtB (2/96,2.08%), two isolates were found to possess the qnrS, oqxA, oqxB,aac(6’)-Ib-cr and blaTEMgenes in the same time. PMQR factor in combination with β-lactamase were dominatedby oqxA, oqxB to blaTEMor aac(6’)-Ib-cr, qnrS to blaTEM, PMQR factor in combination with16S rRNAmethylase was dominated by oqxA or oqxB to rmtB.By the above results can be concluded that Escherichia coli isolates from different animal farms showeddifferent degrees of resistance to antibiotics in XinJiang, PMQR factor mediated bacterial resistance Exist inthese farms, and are dominated by oqxA, oqxB gene.In addition, different types of drug-resistant genes cancoexist, This will inevitably increase the level of resistance and lead to the spread of multi-drug resistance risk.