Study On The Effect Of Erchen Decoction On The Expression Of LPL And EL In Obese Rats

Objective:In order to preliminarily reveal the molecular mechanisms of Erchen Decoction associated with regulation of lipid metabolism and therapy for obesity, we detected the changes in mRNA and protein levels of lipoprotein lipase(LPL) and endothelial lipase(EL) of obese rats treated with Erchen Decoction. According to this study, we hoped to supply some experimental bases for Erchen Decoction’s basic research and clinical applications.Method:High Fat Diets induced animal models of obesity were generated in Wistar rats. The Wistar rats models of obesity were randomly divided into3groups (n=10per group):Model group, Erchen Decoction group and Metformin group. Other10normal Wistar rats fed with common diets acted as normal control (Normal group). The Erchen Decoction group and the metformin group were treated with intragastric administration of Erchen Decoction or metformin respectively for4weeks. The model group and the normal group were treated with intragastric administration of equal volume of distilled water. After the experiment, serum biochemical parameters in rats were detected with automated biochemical analyzer and the mRNA and protein levels of LPL, EL in liver and adipose tissues of rats were detected with RT-qPCR or western blot.Result:1. Changes in body weight, body length and abdominal circumference. The body weight, body length and abdominal circumference of rats in the model group increased significantly (P<0.05) compared with the normal group. And the indices in the Erchen Decoction group and the metformin group reduced compared with the model group (P>0.05).2. Changes in serum biochemical parameters.The levels of TC,TG and LDL-C of rats in the model group increased significantly (P<0.05) compared with the normal group. The levels of TG and LDL-C in the Erchen Decoction group decreased significantly (P<0.05) compared with the model group. The levels of TG and TC in the metformin group decreased significantly (P<0.05) compared with the model group.3.Changes in the expression of LPLmRNA in adipose tissue and ELmRNA in liver tissue.The expression of LPLmRNA in adipose tissue in the model group increased compared with the normal group (P>0.05). The expression of LPLmRNA in adipose tissue in the Erchen Decoction group and the metformin group increased both compared with the model group (P>0.05).The expression of ELmRNA in liver tissue in the model group decreased significantly compared with the normal group(P<0.05). The expression of ELmRNA in liver tissue in the Erchen Decoction group and the metformin group decreased significantly compared with the model group(P<0.05).4.Changes in the expression of LPL protein in adipose tissue and EL protein in liver tissue.The expression of LPL protein in adipose tissue in the model group increased significantly compared with the normal group (P<0.05). The expression of LPL protein in adipose tissue in the Erchen Decoction group and the metformin group increased significantly compared with the model group (P<0.05).The expression of EL protein in liver tissue in the model group decreased significantly compared with the normal group(P<0.05). The expression of EL protein in liver tissue in the Erchen Decoction group and the metformin group decreased significantly compared with the model group(P<0.05).5. There were no statistically significant difference in the expression of LPL and EL between the Erchen Decoction group and the metformin group (P>0.05).Conclusion:Erchen Decoction could decrease the levels of TG and LDL-C in serum of obese rats. Upregulating the level of LPL protein in adipose tissue and downregulating the level of EL protein in liver tissue were the possible mechanism.