Establishment Of Rats Models Of Traumatic Hemorrhagic Shock Under Dry Heat Environment Of Desert And Influence Of Dry Heat Environment Of Desert On Liver Injury

Traumatic hemorrhagic shock(THS) is due to the body after violent action, the important viscerainjury, severe bleeding, etc., make the effective circulating blood volume in patients with reduced,microcirculation perfusion inadequacy, and posttraumatic acute pain, fear, formed by many factors such asthe comprehensive body of decompensated syndrome. Since half a century most of the local war andplateau, cold, hot, desert, island and other special geographical environment. Gobi and desert areas hasobvious summer high temperature, large temperature difference between day and night, dry, hot, drysummer climate has increased the heat stress response of the body, when the body by trauma can inducethe occurrence of shock, increase the difficulty of the subsequent rehydration therapy and mortality. Thisexperiment at normal environment and dry heat environment of desert, the establishment of traumatichemorrhagic shock model of rats and explore the dry heat environment of desert of traumatic hemorrhagicshock mechanism of liver damage, for laying a foundation for the further research of traumatichemorrhagic shock.Objects:(1) To establishment a rats models of traumatic hemorrhagic shock under dry heat environment ofdesert.(2) To observe the liver pathologic histology, hepatocyte ultrastructure and the change of function intraumatic hemorrhagic shock rats under dry heat environment of desert, and explore the characteristic andrule of liver damage.Methods:(1) To control male SD rats reach traumatic hemorrhagic shock caused by wound and carotidartery auxiliary bloodletting under dry heat environment of desert, make the mean arterial pressure (MAP)of rats reached35±5mmHg level, establish a rats models of traumatic hemorrhagic shock under dry heatenvironment of desert.(2) A total of140male SD rats were equally divided randomly into traumatichemorrhagic shock group under normal environment and traumatic hemorrhagic shock group under dryheat environment group, and each group were equally divided into7subgroup: control group and shock0h group,0.5h group,1h group,1.5h group,2h group,3h group.The model of traumatic hemorrhagicshock was established,at the corresponding time point, rats were executed and the specimen wereobtained,the change of liver pathology, hepatocyte ultrastructure under electron microscope were observedand the levels of Serum alanine aminotransferase(ALT)、aspartate aminotransferase(AST) were measured.Results:(1) The survival of normal environment group and dry heat environment groupⅠwas obviouslyhigher than that of dry heat environmental groupⅡ（P<0.01),Pathology examination showed that tissue ofheart, lung, and liver appeared tissue edema, degeneration, leukocyte infiltration, Extensive bleeding,necrocytosis is more serious in dead rats of normal environment group and dry heat environment groupⅠand dry heat environment groupⅡrats.(2)After traumatic hemorrhagic shock,The lesions of liver tissue structure were more serious in dry heat environment group than the corresponding time point of normalenvironment. And also the tissue compensatory adaption were advanced in dry heat environment.Levels ofserum alanine aminotransferase(ALT)、aspartate aminotransferase(AST)、Interleukin-6(IL-6) and TumorNecrosis Factor alpha(TNF-α) increased significantly and appeared dynamic change,and its had the similarchanging trend as the change of Liver pathology morphology. Electron microscope results indicated thatthe damages of hepatocyte mitochondria were more serious in dry heat environment group than normalenvironment group.Conclusions:(1)The success of the experiment established the rats model of traumatic hemorrhagic shockunder dry heat environment, and show that dry heat environment can obviously reduce the survival rate oftraumatic hemorrhagic shock rats, it should be immediately transfer to send after injury.(2)Dry heatenvironment of desert traumatic hemorrhagic shock liver damage occurs earlier and more serious thannormal environment. Hepatocyte mitochondria damage may play an important role in the process of liverinjury.