Effects Of SPV On Intestinal Redox Of Severe Traumatic Hemorrhagic Shock In Rats

Objective: To observe the intervention of Smecta, Polymyxin B, and Vitamin B6 (SPV) on the intestinal redox in rats with severe traumatic hemorrhagic shock by establishment of traumatic hemorrhagic shock model of rats and measurement of serum changes of MDA, SOD, and to evaluate the possible mechanism of SPV on intestinal preservation in traumatic-hemorrhagic shock.Materials and methods: 88 healthy male SD rats were divided randomly into control group (no special treatment, n=8), shock group (traumatic-hemorrhagic shock model, n=40), and SPV group (traumatic hemorrhagic shock model with intervention of SPV, n=40). The shock group and SPV group were subdivided into 5 subgroups of 1h, 2h, 4h, 8h and 16h after set-up of the shock, with 8 animals in each time point subgroup. The serum levels of MDA, SOD were examined by colorimetric method, and the morphological observation of intestinal mucus damage was examined and evaluated with optical microscope and Chiu's score. Experimental data were analyzed using single factor of variance, P < 0.05 as significant difference, P < 0.01 as highly significant difference. SPSS 17.0 statistical software was applied for statistical calculation.Result: 1. The intestinal mucosa damage index of shock group rose early and rapidly. The pathomorphological changes of intestinal mucosa were aggravated with shock prolonged in shock group. In SPV group, the intestinal mucosa damage index had also an obvious increase early but slowly, and had a sharp drop at 4h after shock, with the better improvement obviously and significantly at 8 and 16h subgroup, compared with the same time point of shock group (P < 0.05).2. The serum MDA contents increased obviously in shock group at 1h after severe traumatic hemorrhagic shock, and continued to rise with the time prolonged, compared with control group (P < 0.01 at all time points); In SPV group, serum MDA contents increased slowly, peaked at 4h after shock, and decreased significantly at 16h, (P < 0.01), reached to about the level of 1h subgroup (P > 0.05). The serum MDA contents were significantly less at all time points of SPV group than those of shock group (P < 0.05).3, The serum levels of SOD vitality declined continuously along with the shock prolonged, with the lowest at 16h after shock in shock group (P < 0.05, compared with controls at all time points). In SPV group, the serum levels of SOD vitality declined slowly and slightly in the early time, and kept higher from 2h to 16h after shock than those in shock group (P < 0.01). The serum SOD vitality of SPV group dropped to the lowest at 8h, and it went back at 16h to the level close to that 1h after shock (P > 0.05, compared 16h with 1h subgroup).Conclusion: 1. SOD activity and MDA level change in the opposite directions during the shock. SOD is consumed and its activity decreased, resulting in the decrease of clearance function of OFR, which in turn lead to further increase of MDA. Therefore, MDA reflects the damage degree of body cell attacked by OFR; SOD reflects the level of the body's ability to eliminate OFR. The detection of both SOD and MDA can better reflect the production and removal of OFR.2. The longer the intestinal ischemia lasts, the lower the serum SOD activity in rats. Intestinal ischemia may trigger the proliferation of free radical chain reaction and produce a large amount of toxic OFR, leading to tissue damage.3. SPV may reduce SOD consumption and improve the OFR clearance, so as to protect the intestinal mucosa in traumatic-hemorrhagic shock rats.