The Expression And Significance Of PPARβ And ANGPTL4in Different Cervical Tissues

Objective: To study the expression of PPARβ, ANGPTL4innormal cervix, cervical intraepithelial neoplasia(CIN) and cervical carcinoma,and to investigate the possible mechanism of them in the development andprogression of cervical cancers. Methods: Immunohistochemistry (IHC)Envision was used to measure the expression of PPARβ and ANGPTL4in38cases of cervical carcinoma,10cases of normal cervical tissues and11cases ofCIN tissues including3cases of CIN I,3cases of CIN II and5cases of CIN III.This study was to analyze the correlation among the expression of PPARβ,ANGPTL4and the clinicopathological parameters so as to investigate thepossible mechanism and their relationship in the development of cervical cancer.Chi-square test or Fisher’s exact test was used to detect the experimental results.Spearman rank correlation analysis was used to analyze their correlation. Thetwo-sided test was applied and P<0.05was recognized statistically significant.Results:1. PPARβ was mainly located in the cytoplasm in normal cervix, CINand cervical cancer, sometimes in the nucleus. ANGPTL4was cheifly locatedin cytoplasm in normal cervix, CIN and cervical cancer tissues.2. The positiveexpression rates of PPARβ were gradually increased with10%(1/10),54.55%(6/11),86.84%(33/38) in normal cervix, CIN and cervical carcinomarespectively and their differences had statistical significance(χ2=22.498,P<0.001). The comparison results among three groups showed that there wasno statistically significant difference between normal cervix group and CINgroup (χ2=4.677, P=0.063>0.05) while there was statistically significantdifference between CIN group and cervical cancer group (χ2=5.478,P=0.033<0.05) so was in the normal cervix group and cervical carcinoma group(χ2=22.626, P<0.001). No associations were found between the expression of PPARβ and the clinical pathological indexes including the age of the patients,FIGO staging, the depth of stromal invasion, histological type, vascularinvasion, lymph node metastasis, tumor size and the degree of differentiation incervical cancer.3. ANGPTL4was chiefly located in cytoplasm in normalcervix, CIN and cervical cancer.4. The positive expression rates of ANGPTL4were10%(1/10),36.36%(4/11),71.05%(27/38) in normal cervix group, CINgroup and cervical carcinoma group respectively which increased gradually andthe differences had statistical significance (χ2=22.498, P<0.001). Thecomparison results among three groups showed that there was statisticallysignificant difference between the normal cervix group and the cervicalcarcinoma group(χ2=12.141, P=0.001<0.05) while there was no statisticallysignificant difference between normal cervix group and CIN group(χ2=2.077,P=0.311>0.05), so was in the CIN group and cervical cancer group(χ2=2.077,P=0.311>0.05). There was a higher ANGPTL4expression trend in the deepmuscular layer and layer invasion(75.76%) than the shallow ones(40%),so as inthe vascular invasion groups(100%) than not(63.3%). However, no associationswere found between the expression of ANGPTL4and the age of the patients,FIGO staging, the depth of stromal invasion, histological type, vascularinvasion, lymph node metastasis, tumor size and the degree of differentiation incervical cancer.5. No associations were found between the expression ofPPARβ and ANGPTL4in cervical cancer (rs=0.267, P=0.106). Conclusion:1.PPARβ was chiefly located in the cytoplasm in normal cervix, CIN and cervicalcancer, sometimes in the nucleus. ANGPTL4was chiefly located in cytoplasmin normal cervix, CIN and cervical cancer.2. The expression of PPARβ andANGPTL4in normal cervical tissue, CIN and cervical carcinoma showed anincreasing trend, which indicated that both may be associated with thedevelopment and progression of cervical cancers.3. No associations werefound between the expression of PPARβ and the age of the patients, FIGOstaging, the depth of stromal invasion, histological type, vascular invasion, lymph node metastasis, tumor size and the degree of differentiation in cervicalcancer, so were in the expression of ANGPTL4.4. No associations were foundbetween the expression of PPARβ and ANGPTL4in cervical cancer, whichindicated that the pathway induced by the two factors may be different in thedevelopment and progression of cervical cancers.