Studies On The Optimal Enzymolysis And The Inactivation Process Of The Scorpion And Its Anti Tumor Effects

Objective:This study studies the optimal enzymolysis process of scorpion, the best inactivated process of scorpion powder, do cellular experiment by MTT and observe the reaction of tumor-bearing mice to scorpion powder, as a result this animal traditional Chinese medicine can get better development and utilization.Methods:Using Coomassie brilliant blue method and the Folin phenol method to determine protein content in order to measure enzymolysis effect;exploring the best enzymolysis process by orthogonal experiment;using MTT method in vitro anti-tumor experiment to observe the inhibition of scorpion extract to the growth of breast cancer cells, esophageal cancer cells, lung adenocarcinoma cells and normal cells;exploring the anti-tumor effect of scorpion preliminarily by using tumor bearing mice spleen index, thymus index, MDA content and SOD activity in serum as the goal.Results and conclusion:According to the formula of the relative content of active oligopeptide, setting the oligopeptide content before fermentation and enzymolysis to zero,so oligopeptide content after fermentation before enzymolysis, before fermentation after enzymolysis, after fermentation after enzymolysis were8.15,4.63and7.36(unit:m/g*100g-1) respectively, which indicates that the oligopeptide content after fermentation before enzymolysis is highest, the active oligopeptide content in the baking powder significantly increased.The orthogonal experiment shows that in the simulated gastric extraction, when the ratio of enzyme to substrate was2.00%, pH was2, enzymolysis time was60min, the protein content is highest,93.06mg/g;in simulated intestinal extraction, when the ratio of enzyme to substrate was5%, pH was8.5, enzymolysis time was360min, the protein content is highest,50.61mg/g.The scorpion aqueous extract of the optimal enzymolysis process, when pH was1.5, the temperature was60℃, inactivation time was30min, the inactivation process was optimal.The scorpion extract before fermentation and enzymolysis, after fermentation before enzymolysis, after fermentation and enzymolysis have different degrees of inhibitions on esophageal cancer cells, breast cancer cells and lung adenocarcinoma cells. Among them the effect of the extract after fermentation before enzymolysis in inhibiting the growth of lung adenocarcinoma cells was especially obvious.Scorpion aqueous extract can inhibit the growth of mouse S180sarcoma cells, increase the spleen index and thymus index, significantly increase the activity of SOD, which suggested that the anti-tumor effect of scorpion extract was related to reducing the lipid peroxidation in vivo, eliminating the excessive harmful free radicals in vivo, and strengthening the" immunity of organism.