Impact Of Shuanghuang Xiaocuo Pill On Rabbit Ears Acne Models Of Plasma IL-8, TNF-α Content

The study of Chinese medicine theory，combined withmodern medical research on acne，to explore ShuanghuangXiaocuo pill acne elimination mechanism of action on animalexperiments. Shuanghuang Xiaocuo pills treated thousandsof patients to explore an effective drug for treating acne.Experimental study on rabbit ears by now acne animal modelof the immune system，to further validate the role ofShuanghuang Xiaocuo pills on the immune system，providedetailed experimental data for good clinical efficacy，in order to verify its mechanism of action for the treatmentof acne and efficacy，in order to further develop a newgeneration of high efficiency，low toxicity，new Chinesemedicine economic foundation.Objective： To study t Shuanghuang Xiaocuo pilleliminate acne and acne treatment effects on animalexperiments acne rabbit ear model of plasma IL-8，TNF-αlevels trends，and explore the acne elimination mechanismof action，clinical provide a theoretical basis for thetreatment of acneMethods：48male New Zealand white rabbits with12weeks， randomly divided into six groups: ShuanghuangXiaocuo high dose group， middle dose group and low dosegroup， positive control group， model control group，blankcontrol group8. In addition to the control group，toestablish an animal model of rabbit ear acne: the methodaccording to the literature about learning to operate ineach ear canal opening in the rabbit ear2cmx2cm range，1day painted tar times O.5mL，for two consecutive weeks The first14days in normal animals and modeling conductedrandomly selected10rabbit ear skin biopsy，tissue blockswith10%formalin-fixed，paraffin-embedded sections foreach specimen serial sections4，HE staining，observed undera microscope to determine the successful model，the animalsin each group orally administered once a day for14days.Respectively，before and after administration of blood，serum IL-8，TNF-α levels by radioimmunoassayResults：The detection of serum levels before and aftertreatment in vivo animal models to prove ShuanghuangXiaocuo on animal models IL-8，TNF-α levels trends provespecific regulation of d Shuanghuang Xiaocuo eliminateacne on the immune system.Conclusion: Compair of Shuanghuang Xiaocuo high-dosegroup，medium and low-dose group and the positive controldrug Tanshinone capsule were to make the animal model IL-8，TNF-α levels decreased...