On The Blood Glucose And Leptin In Type2Diabetic Rats

Objective: Diabetes mellitus has become a serious global public health problemthat threatens the human health. Previous studies have found Roux-en-Y gastricbypass(RYGB) operation which could be used to treat obesity in the past can makepatients with type2diabetes mellitus lose weight at the same time the symptoms ofdiabetes can be partially or even completely remitted, with its mechanism not fullyunderstood. This study discusses the role of hepatic insulin resistance playing in thepathogenesis and the molecular mechanism of type2diabetes mellitus and the effect ofgastric bypass on hepatic insulin resistance by observing the therapeutic effect of gastricbypass on type2diabetes rats and the change of the adiponectin and leptin levels inserum and liver. And then it explores the potential mechanism of gastric bypass in thetreatment of type2diabetes.Methods:120Wistar rats were randomly allocated into with surgery group (O group=60rats),sham operation group (groupS=40rats) and control group (group C=20rats). Afterbeing fed high-fat diet for4weeks, O and S groups were intraperitoneally injected STZto manufacture model of type2diabetes. The model rats in two groups were performedthe gastric bypass surgery and the sham operation. Group C were fed a normal dietwithout special treatment.Preoperatively and postoperatively l,2,4weeks detect thechanges of random blood glucose, fasting blood glucose, body weight, serumadiponectin and leptin. Preoperatively and postoperatively4weeks measure the changesof the adiponectin and leptin detection in the liver.Results：1, Compared with the preoperative,the fasting and random blood glucose of thegroup O were obviously decreased in the sencond week after Gastric Bypass(GBP),respectively from19.562.49mmol/L to7.262.07mmol/L (P <0.05) and from 28.762.71mmol/L to11.662.68mmol/L (P <0.05). The fasting and randomblood glucose were stable in the normal range namely4.901.87mmol/L (P <0.05)and10.702.61mmol/L (P <0.05) in the fourth week after GBP.2, The body weightof O group decreased obviously in the fourth weeks after GBP, declined frompreoperative27923.79mg to19912.26mg (P <0.05).3, The serum adiponectinlevels of group O obviously increased in the second week after GBP, frompreoperatively4.581.53μg/ml to7.051.23μg/ml (P <0.05). The result in the fourthweek is7.460.96μg/ml (P <0.05). However, the serum leptin levels in the secondweek after GBP obviously decreased, from5.930.52ng/ml to2.060.49ng/ml (P<0.05). The result in the fourth week postoperatively was1.930.51ng/ml (P <0.05).4, The hepatic adiponectin and leptin of the group O obviously increased in the sencondweek after GBP, respectively from preoperatively0.370.18μg/mg to1.230.42μg/mg(P <0.05) and from preoperatively0.040.02ng/mg to0.360.31ng/mg (P <0.05).But the changes of fasting and random blood glucose, body weight, adiponectin andleptin levels in serum and liver of the S and C groups had no significant differencepreoperatively and postoperatively (P>0.05).Conclusion:Gastric bypass surgery can significantly decrease blood glucose and body weightin diabetic Wistar rats. That postoperatively the serum adiponectin elevated and theserum leptin reduced plays a key role in controlling blood glucose and body weight andis a major cause of relieving type2diabetes mellitus. Meanwhile, gastric bypass surgerycan also produce a good improvement on hepatic insulin resistance by increasingadiponectin and leptin in the liver thus to have an essential positive impact on thetreatment of type2diabetes mellitus.