The Study Of Upsteam And Downsteam Of MicroRNA In Hematopoiesis

MicroRNA is a small non-coding RNA molecule found in plants and animals, which functions in transcriptional and post-transcriptional regulation of gene expression. MicroRNAs were discovered in1993by Victor Ambros, Rosalind Lee and Rhonda Feinbaum during a study of the gene lin-14in C. elegans development. They found that LIN-14protein abundance was regulated by a short RNA product encoded by the lin-4gene. MiRNAs regulate all aspects of a cell, including its differentiation, function, proliferation, survival, metabolism, and response to changes in its environment. Tight control of gene expression is required for hematopoiesis.Accroding to the research we make earily and papers related,we foucs on mir-144/451,mir-142and mir-223.Alough mir-144/451,mir-142and mir-223plays an important role in hematopoiesis,but the mechanism is not clearly known.Through bioinformatics method, we select some upsteam and downsteam candidate genes.And we find that mir-142can target racl gene.Racl is a member of GTPase.During Mel erythroid cells differentiation,mir-142-3inhibite maturation of red cell possibly via targeting racl expression.