Comparative Study Of Guinea Pig’s Gut Morphology And Functional Changes Between Gallbladder Surgery And Conventional Cholecystectomy

Objective: The traditional cholecystectomy has been the preferred method oftreatment of gallbladder disease,1987-Philippe Moure t completed the world’s firstlaparoscopic cholecystectomy,1991-荀祖武etc. in our first reported laparoscopiccholecystectomy. Laparoscopic cholecystectomy has developed rapidly, because with lesstrauma, beautiful, quick recovery, etc. in hospitals above the county level and has becomethe preferred method of conventional cholecystectomy. But after cholecystectomy, somepatients suffered from diarrhea, constipation and other bowel habits and (or) stool changes,and even abdominal pain, loss of appetite, weight loss, heating and jaundice and othersymptoms, it was called biliary surgery syndrome (PCS). And treatment is poor, affectingpatients with normal work, study; and long-term monitoring of patients aftercholecystectomy found an increased incidence of colon cancer, compared to thesequestions so far lacking unity and a clear understanding of this issue at home and abroadreported relatively less, how to reduce postoperative complications as little as possiblewhile addressing gallstones is worth studying the problem. The experiment by comparingthe guinea pig gallbladder retained after conventional cholecystectomy and Paul gall stonesurgery bowel movement, the difference intestinal morphology, looking forcholecystectomy diarrhea, increased incidence of colon cancer causes and prevention ofsome theoretical basis, explore the significance of gallbladder surgery.Methods: lithogenic diet feeding clean healthy guinea pigs60, weight230-280g,were randomly divided into A (gallbladder surgery), B (cholecystectomy)2groups were30. A group of incision gallbladder, depletion stones, cystic duct ligation group B,cholecystectomy, sterile surgical principles are followed, the same anesthesia,postoperative1week wound healing, normal diet; after4weeks, fasting12h, forbidden todrink2h,intraperitoneal injection of anesthesia (anesthesia), open under sterile, sterile conditions guinea pig abdominal cavity, stomach and cecum found injecting the sameconcentration of barium sulfate suspension2ml in the antrum and cecum place, close theabdominal cavity,30min after, the guinea pigs were killed dislocated neck, open theabdominal cavity, cut and remove the gastrointestinal tract, mesenteric cleared, the entirebalance of intestinal5g weights hung vertically to measure the whole intestinal length andthe length of the small intestine and colon, respectively; with Helicobacter (H. ring),ileocecal (ileocecal valve) as a starting point, measure and record the movement of bariumsulfate in the small intestine and colon in the distance, calculate the percentage of thecorresponding barium sulfate guinea pig small intestine and colon movement distanceaccounted for the whole intestine, the results compare two groups of guinea pigs. Andsmall intestine were collected (from the pylorus20cm, from the ileocecal valve10cm) andcolon (from the ileocecal valve10cm, from the anus15cm) intestinal tissue a lightmicroscope to measure the jejunum, ileum, colon thickness and jejunum, ileum villusheight, width.All data were expressed as mean±standard deviation (xˉ±s) that the groups werecompared using the t test, Using the SPSS16.0statistical analysis software.The result:1.Comparison between groups of barium sulfate in the small intestine propulsion rate(percentage): A group:35.05±2.56, group B:43.14±3.03, between group B and group A,the difference was significant (P <0.05).2.in each group advance rate of barium sulfate in the colon (percentage) Compare: Agroup:5.15±3.14, group B:7.53±2.89, between group B and group A, the differencewas significant (P <0.05).3.light microscope Group B intestinal villous atrophy, crypt deepened, no glandhyperplasia, a small amount of lamina propria lymphocytes, submucosal lymphoid nodulesscattered, from high columnar epithelial cells grow shorter, partly cubical; colonic mucosathinner, epithelial integrity, non-proliferation of goblet cells, lamina propria lymphocyteinfiltration see.4.1jejunum villus height: A group was264.32±35.91μm, group B was239.47±28.51μm, the difference was significant (P <0.01).4.2jejunum villus width: A group was71.46±14.71μm, group B was62.36±11.35μm, the difference was significant (P <0.01). 4.3jejunal mucosal thickness: A group342.16±55.32μm, group B315.82±49.42μm,the difference was significant (P <0.01).4.4ileal villus height: A group was234.24±25.51μm, group B was219.47±22.07μm, the difference was significant (P <0.01).4.5ileal villus width: A group was75.46±18.37μm, group B was68.36±15.39μm,the difference was significant (P <0.01).4.6ileal mucosa thickness: A group412.16±40.56μm, group B401.82±42.72μm,the difference was significant (P <0.01).4.7colonic mucosa thickness: A group was432.61±42.38μm, group B was420.27±53.22μm, the difference was significant (P <0.01).Conclusion: after gallbladder removal intestinal intestinal contents propulsionenhancements, mild atrophy of intestinal epithelial cell microvilli narrow, affect theintestinal absorption, which may be thinning postoperative stool, increased frequency ofreasons.