Rhubarb Decoction Effective Component Change And The Study Of Intestinal Peristalsis Function In Mice

OBJECTIVE:Concluding the original,the chemistry component,the pharmacological efficiency clinical application of the rheum officinale.Boiling and extracting on rheum officinale to measure the amount of the main component variety as the time going in three anthraquinones,combined anthraquinones,tannin.asuring the the relationship between the diarrhoea function and the efficiency component through the intestinal propulsion experiment.METHOD:Extracting samples in different time point in 240 minutes,filling the same amount of water back.making each sample solution purify,hydrolysis,coloured and getting the the comparative amount of free anthraquinones?combined anthraquinones,tannin.Through the intestinal propulsion experiment,grouping the 80 Kunming mouses into normal group,boiling only rheum officinale for 20 minutes?boiling only rheum officinale 180 minutes boiling only for rheum officinale for 180 minutes eliminating the tannin with alcohol.traditional boiling method,three ones boiling together,boiling together without tannin,the percentage of pushing in intestine on each one feeding medicine soup.RESULTS:the amount of combined anthraquinones,free anthraquinones,tannin is increasing added as the time going,and reach the peak at the 120 minute.combined anthraquinones change into free anthraquinones as the time going and proved not to be termal resistor.we found that the reason why boiling rheum officinale in short time is that the huge amount of tannin resisting the diarrhoea efficiency.The test in the Large Chengqi Decoction without tannin proved that the main reason on decreasing efficiency in diarrhoea is the amount of tannin coming out.Providing meaningful reference to the development of new drug style and tannin clinical application,renewed the traditional opinion in the reason of making rheum officinale diarrhea.