Relation Between Line-1Methylation And Non-small Lung Cancer

Objective This paper aims to study the relationship between line-1methylation level and clinical characteristic, living habits as smoking and environmental exposures of non-small cell lung cancer patients. Methods Pyrosequencing was used to detect the line-1methylation level of lung cancer tissues and adjacent tissues of197primary non-small cell lung cancer patients. And non-conditional logistic multivariate regression model was used to analyze the relationship between line-1methylation level and clinical characteristics, living habits as smoking and environmental exposures of non-small cell lung cancer patients. Results Methylation levels were68.20±11.63and78.90±2.09(P<0.001) respectively for the cancer tissues and adjacent tissues. Non-conditional logistic model built based on epidemiologicalinformation showed that the line-1methylation levels of squamous cell carcinoma and other types of non-small cell lung cancer patients were significantly lower than patients with adenocarcinoma, OR was8.82(95%CI:2.00～38.96) and26.68(95%CI:3.04-234.04); Line-1methylation level of peripheral lung cancer patients was significantly lower than central lung cancer patients, OR=9.17((95%CI:2.15～39.10); line-1methylation level was more likely to decrease for mild smoking patients than for non-smoking patients, OR=17.58(95%CI:0.64～89.58), No significant difference; heavy line-1 methylation levels were lower than non-smokers smoking patients, OR=8.17(95%CI:1.40-47.54). After fixing the age, gender, family history of cancer, the control variable P>0.1factor into logistic regression model for further analysis, The results showed that, line-1methylation levels in patients with squamous cell lung cancer and other types of non-small cell cancer patients below, OR values were6.29(95%CI:1.72～22.98) and14.60(95%CI:2.07～103.23); Line-1methylation levels in patients with peripheral lung cancer patients with central lung below, OR=7.27((95%CI:1.97～26.85); mild line-1methylation levels may be lower in patients with non-smoking patients with pharyngeal suction, OR=8.51(95%CI:0.76～95.80), no significant difference; heavy line-1methylation levels were lower than non-smokers smoking patients, OR=5.47(95%CI:1.19～25.07).