Studies On The Chemical Constituents And Biological Activity OfEuphorbia Antiquorum

This dissertation is composed of four chapters. Chapter1reviewed recent progress on the distribution, pharmacodynamics, and chemical composition of the medicinal plants of the genus Euphorbia. The investigation on chemical constituents and bioactivities of two different habitats Euphorbia antiquorumwere reported in chapters2and3. Chapter4was a conclusion of this dissertation.By the means of chromatographic methods and spectroscopic evidences,24compounds were isolated and identified from two different habitats E. antiquorum, including diterpenoids (atisane, kaurane andabietane), liganas, triterpenoids, coumarin and sterols compounds. Among them, three compounds were new ones and one compound was new natural product. Phytochemical investigation on the two different habitats of E. antiquorum indicated that the major consitituents of these two plants were diterpene compounds,especially atisane diterpenes; the content of titerpenoidswas great, but the structureswere short of diversity. In addition, two plants both containded coumarin compounds.E. antiquorum belongs to the genus Euphorbia (Euphorbiaceae). Most of them growth near the cottage or garden, and cultivated of ornamental or hedgerow plants. Theorigin of this plant were India and Sri Lanka, and it is mainly distributed in Yunnan Province, Sichuan province and other places of China. It has long been used in traditional Chinese Dai medicine. The stems of E. antiquorumwere used for the treatment of swelling, acute gastroenteritis, inflammatory swelling, scabies, epilepsy, ulcers and skin diseases; their leaves wereused for the treatment of tropical diarrhea, rash abusive, cramps and cholera. Thus, our investigation on the chemical constituents of the leaves and stems of this plant collected in two differert habitats (Xishuangbanna and Dehong, Yunnan Province) resulated in the isolation of two new compounds, a new natural product, together with21known compounds.Atisane-type diterpenoids isolated fromE. antiquorum collected in Xishuangbannan, were evaluated for theirinhibitionof cell proliferation. The results showed that compound 2(3-oicacid methyl-agallochaol C) showed moderateinhibition of cellproliferation.