Studies On The Chemical Components In Seeds Of Euphorbia Lathyris

Euphorbia L.is the largest genus of Euphorbiaceae.Most plants of this genus contain a milky irritant latex,and the whole plants roots,stems,leaves,seeds and latex of them were investigated in chemical and pharmacological studies.Diterpenoids,the main active constituents of Euphorbia?E.?plants,are the focus of natural product drug discovery due to the complex structure and various biological activities.E.Semen is the dried ripe seeds of E.lathyris L..Studies have shown that the diterpenoids,sterols,coumarins of E.semen have anti-cancer,anti-inflammatory,analgesia,whitening activities.In order to better develop and use medicinal resources of E.plants and enrich the chemical composition of E.semen,the chemical composition were studied systematically.The total CH₃CN extract of E.semen was obtained by EtOH soaking and Petroleum ether-CH₃CN extraction.And then,27 compounds were isolated by varing separation methods like chromatography and recrystallization.Their structures were elucidated by extensive spectroscopic analyses and literature comparison.Among them,seven new compounds were determined as Euphorbia Factors L₂₇-L₂₈?1-2?,Premylanin?3?,Euphorbia Factors L₃₀-L₃₃?4-7?.Twenty known compounds were identified as Euphorbia Factor L₁?8?,Euphorbia Factor L₂?9?,Euphorbia Factor L₃?10?,Euphorbia Factor L_7a?11?,Euphorbia Factor L_7b?12?,Euphorbia Factor L₈?13?,Euphorbia Factor L₉?14?,Euphorbia Factor L₁₀?15?,Euphorbia Factor L₁₁?16?,Euphorbia Factor L₁₂?17?,Euphorbia Factor L₁₅?18?,Euphorbia Factor L₁₇?19?,Euphorbia Factor L₁₈?20?,Euphorbia Factor L₂₃?21?,Euphorbia Factor L₂₄?22?,Euphorbia Factor L₂₆?23?,Lathyranoic acid A?24?,Epoxyboetirane A?25?,Piscatoriol B?26?,Euphoscopoid C?27?.Furthermore,the anti-cancer activity of compounds1-6,8-27 was investigated by MTT assay,which showed compound 2 had strong inhitory effects on MCF-7 and HepG2 cancer cells and compound 22 had significant inhitory effects on HCT-116,MCF-7,786-0 and HepG2 cancer cells.In addition,384 diterpenoids isolated from E.plant in recent six years and their physiological activities were also summarized.