Research On Infection Of Kaposi’s Sarcoma-associated Herpesvirus In Children Of Parts Of Xinjiang And Risk Factors

Objective: In order to investigate the prevalence of Kaposi’s sarcoma-associated herpesvirus (KSHV)infection status in children of Xinjiang, explore the infection way and risk factors in Children withKSHV. So as to reduce the spreading of children with KSHV and prevented the spread of KSHV inpublic health.Method:1. Caregivers have children between6and60months of age, attending local clinics in Xinyuan andJiashi Counties in Xinjiang province, were approached to participate in this study. Using questionnairesto collect general information, including general information, breastfeeding and eating behavior, familystructure, living conditions, lifestyle, etc. There were323donors had been detected, included171children and152caregivers.2. Using the monoclonal-enhanced immunofluorescence assay (mIFAs) technology tested KSHVantibody in plasma samples.3. Using nest PCR detect DNA ORF26and GB gene of Peripheral blood.4. Combined with the statistical data, using SPSS17.0software for statistical analysis the twoclassification Logistic linear regression analysis between the factors and children’s virus positiverelationship, for P <0.2factors of multiple factors regression analysis, inspection level of alpha=0.05,the difference was statistically significant(P≤0.05). In order to confirm the relationship between thefactors and KSHV positive, conform the risk factors of children infected with KSHV.Result:1. Serum antibody test results showed that KSHV seroprevalence in children and caregivers in Xinjiangwas51.5%and66.5%.Logistic regression to single factor analysis found that children eating hard foods(OR=1.3695%CI=1.15-3.86; P=0.02), and fed the children with chewed food (OR=2.7195%CI=1.32-8.55; P=0.004) also had statistical significance. Two significant associations were observedthat child KSHV seroprevalence with children eating hard food and feeding the children withchewed food by family members. Also, logistic regression multivariate analysis found that childreneating hard foods (OR=2.6195%CI=1.06-6.42; P=0.04), and fed the children with chewed food(OR=5.6595%CI=1.58-13.75; P=0.005) also had statistical significance. Two significant associationswere observed that child KSHV seroprevalence with children eating hard food and feeding the childrenwith chewed food by family members.2. DNA test results showed that mothers were20.4%(31/152); Children were12.9%(22/171).Logisticregression to single factor analysis found that children eating hard foods (OR=2.3295%CI=1.86-6.26;P=0.03), and family members testing temperature of food(OR=3.3995%CI=1.15-11.02; p=0.05) also hadstatistical significance. Two significant associations were observed that child KSHV seroprevalence withchildren eating hard food and family members testing temperature of food. As well as, logistic regressionmultivariate analysis found that children eating hard foods (OR=2.5695%CI=1.41-4.88; P=0.03), andfamily members testing temperature of food (OR=3.4395%CI=2.10-15.02; P=0.02) also had statisticalsignificance. Two significant associations were observed that child KSHV seroprevalence with childreneating hard food and family members testing temperature of food.Conclusion:1. The prevalence of KSHV infection status in Xinjiang children got a higher positive rate. KSHVinfections occurred in early childhood. And, the higher positive rate is closely related to the high positiverate of their mothers.2. Children eating hard food，fed the children with chewed food and family members testing temperatureof food is KSHV is closely relative to the positive. These were children KSHV infection risk factors,saliva may be an important way of KSHV childhood infection.