The Dynamic Evolution Of Growth Factor In Lung Tissue Of Chronic Obstructive Pulmonary Disease Model Rats Of Syndrome Of Accumulation Of Cold Fluid In Lung

Objective:According to the study of the natural fund project prior, for chronic obstructivepulmonary disease (chronic obstructive pulmonary diseases, COPD) and cold drinkaggregates lung theoretical exploration and classification in the lung tissue growth factorVEGF, TGF-β1dynamic evolution as the main target of observation, explore pathogenesisof COPD cold drink aggregates lung syndrome and dynamic evolution and the lung tissuegrowth factor VEGF, TGF-β1relationship, to enrich and develop COPD cold drinkaggregates lung syndrome theory. Continue this subject adopts cold stimulation methodcombined with smoky and endotracheal instillation of endotoxin (LPS) in establishing colddrink aggregates lung COPD rat model, through observing model rats natural evolution in60days and the wen yang hua yin fang, budesonide intervened lung tissue growth factorVEGF, TGF-β1pathogenesis evolution, to illustrate the COPD cold drink aggregates lungmodel of rat lung tissue growth factor (VEGF, TGF-β1, lung function and lunghistomorphology dynamic evolution, and drink from this side of cold drink aggregates lungCOPD model rat lung tissue growth factor (VEGF, TGF-β1intervention effect, todetermine from the effect and timeliness of wen yang hua yin fang, disease prevention andcontrol to provide new train of thought and theory support for this.Methods: By usingcombination of smoke and the endotracheal drip LPS, drinking ice water (0℃refrigeratorfrozen into ice water) copied into a cold drink aggregates lung disease syndrome COPDrats model, to observe (including interval of10days, a total of60days) under the naturalstate of the model of dynamic evolution mechanism, with using wen yang hua yin fang、budesonide on the model for dynamic intervention, the disease and further observation ofthe general conditions, the model lung histology, lung function testing related indicators,the lung tissue growth factor VEGF, TGF-β1.Results: This experiment is on the basis ofprevious subject in improving and copying the cold drink aggregates lung COPD ratsmodel, the model of form and behavior, index change and cold drink aggregates lungCOPD clinical manifestations. Model rats inhaling resistance， expiratory resistanceincreased, lung compliance reduced, lung tissue growth factor VEGF expression is reduced,TGF-β1expression increases, and the pathological section in inflammatory lung tissueinjury and fibrosis. In removing cold stimulation factors, within60days after symptoms ofmodel rats, the indicators improved significantly, and has the trend of deteriorating, colddrink aggregates lung in rats in about40days began to weaken, could change other syndrome types. With the equivalent of budesonide intervention, model rats symptoms areimproved, but can’t close to normal level in rats, lung tissue growth factor VEGFexpression is not obvious, TGF-β1also has no significant decrease, pathological biopsyshowed inflammatory damage loss, peaked at about40days, the basic level close tonormal rats. Cloth to Ned intervention model rats symptoms are improved, but higher lungtissue growth factor VEGF expression is not obvious, TGF-β1no decrease also,pathological biopsy shows low inflammatory injury.Conclusion: By using the coldstimulation, smoked and endotracheal drip LPS building of cold drink aggregates lungCOPD rats model is consistent with the clinical trials in animal models, with goodreliability and stability. Phlegm retention is the key form cold drink aggregates lung COPD,lung tissue growth factor VEGF expression is reduced, TGF-β1higher expression closelyassociated with the formation of phlegm retention. Using the explored the drinking partyintervention can increase the lung tissue growth factor in the expression of VEGF and cutthe expression of TGF-β1, cold drink aggregates with COPD pulmonary syndrome curativeeffect affirmation, and timely treatment of the disease syndrome, lung tissue growth factorVEGF, TGF-β1is one of the targets.(Note: this topic is the national natural science fund "cold factors induced by chronicobstructive pulmonary disease, cold drink aggregates pathogenesis evolution of dynamicpulmonary syndrome rats model research" project, number:81173152)...