Liver Research On Mechanisms Of Tongqiao Decoction In Treating Sudden Deafness

Objective: This research in the foundation which combs to the ancient and modernliterature, establishes the clear liver to understand, blood circulation removing extravastedblood, to calm the nerves calmly, the adjustment sentiment will mainly governs the law forthe burst characteristics deafness, the union earlier period clinical medication experience,from draws up the clear liver to understand the soup, and observes the clear liver throughthe animal experimentation further system to understand the soup to the guinea pigaudibility threshold change and with pathogenesis in cochlea microcycle barrier closecorrelation series target like blood NO (nitrogen monoxide), ET (bast element),5-HT (5-hydroxy tryptamine) the content influence, by further discusses the Chinese nativemedicine preventing and controlling burst characteristics deafness the action mechanism.Methods: Uses the magnetic tape to transcribe the4kHz concealed narrow band noisewhich the AA30high-level pure tone audiometer sends out is the noise acoustic source,enlarges after the power amplifier to107dB(A), carries on the experimental burstcharacteristics deafness to the guinea pig to make the mold, divides into stochastically40white red item healthy guinea pigs4groups, namely blank group (normal control group);30model animals divide into stochastically: The model group, the masculine controlgroup(blood government office by the stasis oral group), the treatment group (clear liverunderstands soup group), each group of10, record the ABR threshold value determination,after the execution according to literature method examination guinea pig blood serum NO,ET, the5-HT level.Results:1. ABR threshold determination: four groups of short acoustic response ABRthreshold in Guinea pigs before the experiment had no significant difference (P>0.05).Modeled after the model groups improved the response ABR threshold in Guinea pigs, are significant differences compared with the blank Group (P<0.01), confirmed that modelsuccessfully; empty group response ABR threshold in guinea pigs with no significantchanges before the experiment (P>0.05), model, modeled after the positive controlresponse ABR threshold significantly reduced (p <0.05or0.01), but there were significantdifferences compared with the blank group (P<0.05or0.01) and treatment group responseABR threshold down from model group (P<0.01), no significant difference with the blankgroup (P>0.05).2. the levels of serum NO and ET,5-HT: the treatment group and thepositive control plasma ET levels significantly lower than the model group (P<0.01), nosignificant difference in treatment group compared with the white group (P>0.05), thepositive control group and the treated group still had significant differences (P<0.05).Treatment group and the positive controlled group blood NO level obviously above modelgroup (P<0.01or0.05), treatment group NO level obviously above positive controlledgroup (P<0.05), but and blank group compared to still has significantly sexual differences(P<0.05). made die hou guinea pig plasma5-HT content obviously elevated, and blankgroup compared to has significantly sexual differences (P<0.01);2week filling stomachtreatment ended hou, and model group comparison differences has significantly sexual(P<0.01), and blank group no significantly differences (P>0.05) differences comparedwith the model groups with significant positive control group (P<0.01), but compared withthe blank group and treatment group had significant differences (P<0.01or0.05).Conclusions:1. confirmed in the pathogenesis of sudden deafness ET/NO dynamicimbalance, plasma5-HT (5-HT) rise.2. liver tongqiao decoction can raise serum NOsudden hearing loss in Guinea pigs, ET reduced, restoring both balance while reducingplasma5-HT (5-HT) levels.3. confirms the clear opening, activating blood circulation andremoving blood stasis of liver, tranquilizing, regulate emotion as the primary therapy forsudden deafness.