Study On Polymorphism Of Outer Membrane Protein A Gene Of Pathogenic Yersinia

Yersinia pestis (Y.pestis), Yersinia enterocolitica (Y.e) and Yersinia pseudotuberculosis (Y.p) are pathogenic to human, they all belong to Gram-negative bacteria of Yersinia spp. Yersinia pestis is the pathogen of the plague that is deadly infectious disease, Yersinia enterocolitica can cause human enterocolitis, can also cause some sequelas, such as erythema nodosum, reactive arthritis, endocarditis, and even can cause sepsis to death. Yersinia pseudotuberculosis exist in a variety of animal gut, and less induce to human infection, mainly through eating contaminated food.Currently, the research on the outer membrane protein A of Gram-negative Enterobacteriaceae has proved that the outer membrane protein A is a multifunctional protein, at the same time, as the main antigenic components involved in the adaptive immune response. Our previous research has proved outer membrane protein A of Yersinia enterocolitica has highly immunogenicity. outer membrane protein A of Yersinia pestis has also been shown to be immunogenic, and it has a protective effect to Yersinia pestis infection. In order to study these three pathogenic Yersinia outer membrane protein A (ompA) in the gene level,318Yersinia enterocolitica,97Yersinia pseudotuberculosis and45strains of Yersinia pestis ORF of ompA gene were detected and analyzed. Also putative outer membrane protein A (p-ompA) gene of318Yersinia enterocolitica the were detected and analyzed. The sequencing results showed all170pathogenic strains and84strains carrying ystB gene carried both the ompA and p-ompA genes, the64strains of the biotype1A without the ystB gene only carry either the ompA(7strains) or the p-ompA(22strains) gene, and confirmed the ompA gene and p-ompA gene are highly conserved in pathogenic Yersinia enterocolitica. In non-pathogenic strains (biovar1A), distribution of the two genes and ystB gene were highly correlated, and the sequence showed genetic polymorphism. While only a portion of the strains without ystB gene carried one of the two genes separately. Cluster analysis showed pathogenic strains sequence types are less, very conservative, rather than sequence types of non-pathogenic strains were diversity. All of Yersinia pestis and Yersinia pseudotuberculosis ompA gene showed highly conserved, and all of strains containing the ompA gene. By analyzing polymorphism of these three pathogenic Yersinia outer membrane protein A gene, it can be prompted outer membrane protein A gene is the conservative gene of the pathogenic Yersinia, while the outer membrane protein A may be the pathogenic Yersinia pathogenicity-related protein, this research can provide a basis for further study of the function of the of pathogenic Yersinia outer membrane protein A. Yersinia enterocolitica p-ompA gene is conservative, similar to the ompA gene, suggesting that the p-ompA gene may be functionally related gene of Yersinia enterocolitica.