Molybdenum Toxicity To Mice Testis And The Impact On MAPK Signaling Pathway Of Testicular Tissue

ObjectivesThis experiment was to study different concentrations of sodium molybdate on the toxic effects in mice and sodium molybdate in mice testicular tissue MAPK signaling pathways, for the study of molybdenum poisoning effect on reproductive system of male mice, the mechanism to provide the theoretical basis and significance.Methods60 male kunming mice and randomly were divided into 4 groups.Set up a control group and 3 group:Physiological saline control group(n = 15),Low dose group 20mg/kg(n = 15), middle dose group 60mg/kg(n = 15) and high dose group 100mg/kg(n = 15).Daily feed sodium molybdate lavage canister 0.5 m L per mouse and for 30 days.Observed mice health,and measure the weight of testicular.Production of pathological of mice testis.Detection of serum testosterone concentrations for mice.Detection of testicular tissue expression of antioxidant activity of related enzymes, SOD, GSH-Px, LDH, SDH and MDA.Test mice testis gene m RNA expression of ERK1/2,JNK1/2 and P38 by rt-pcr method.Using SPSS17.0 software system analysis results.ResultsAfter different doses of sodium molybdate infected mice body weight and testicles weight than the control group decreased, with statistical difference(P < 0.05).With the increase of infected doses and dose increasing,sodium molybdate could cause mice serum testosterone levels been low, and with the increased concentration of molybdenum exposure, the content of serum testosterone also decreases(P < 0.05). With the increase of dosage of sodium molybdate mice testicular tissue pathological changes, a high dose of sodium molybdate suite structure of seminiferous tubule disorders, lumen almost disappeared, layer number of sperm cells decreased significantly, clearance increased, large areas of cavitation, organization structure was destroyed, all levels of living sperm cells loosely arranged disorder. Born sperm cell and leydig cell falls off, produce could be observed in pathological cell lysis, the phenomenon of cell rupture.Loss of sperm cells scattered in the fine small tube cavity, seriously blocked the fine small tube cavity.The model group, SOD, gsh-px, LDH, SDH activity was significantly lower than control group(P < 0.05) and MDA activity was significantly higher than control group(P < 0.05).The results were dose dependent.Fluorescence quantitative PCR showed the rise in ERK1/2 m RNA expression, a significant rise in JNK1/2 m RNA expression, a significant rise in P38 lightning gene m RNA expression, the statistical difference(P < 0.05).Conclusions 1.20 mg/kg dose group could cause the toxic effects of male mice, mainly displays in the slow growth rate in mice, mice against testicular development. 2. Sodium molybdate infected dose of 20 mg/mg dose group could reduce the male mice serum testosterone levels significantly, physiological structure destruction of testicular tissue. 3.Dose within the scope of this study, sodium molybdate could reduce the activety of antioxidant enzymes of testicular tissue, significantly reduced the increase of lipid peroxidation products, the testicular tissue anti-oxidation ability drop. 4.This study explores the testicular tissue toxicity in mice induced by sodium molybdate change of MAPK signal pathway.The expression of ERK1/2、JNK1/2 and P38 m RNA were changed in dose dependent, This phenomenon may bedue to the toxicity of sodium molybdate which leads to the change.