Effects And Mechanism Of PRMT2 On The Invasion And Migration Of Breast Cancer MDA-MB-231 Cells

Objective: To investigate the effects and possible mechanism of PRMT2 on the invasion and migration of human breast cancer cells MDA-MB-231.Methods: After the recombination eukaryotic expression plasmids were triumphantly constructed and transiently transfected into MDA-MB-231 cells, some EMT-related proteins were examined by Western blot, so the possible effects of PRMT2 on the invasion and migration of MDA-MB-231 cells could be observed. The stable cell lines MDA-MB-231-PRMT2 were established, and the morphological changes of cells could be observed by microscope, meanwhile the abilities of invasion and migration of MDA-MB-231-PRMT2 cells were analyzed by Transwell invasion assay and Scratch-wound assay, and EMT-related proteins were examined by Western blot, to further prove and analyze the effects of PRMT2 on the invasion and migration of MDA-MB-231 cells. The possible signal pathways were detected by Protein microarray, and some pathway-related proteins were examined by Western blot, to investigate the mechanism of PRMT2 affecting the invasion and migration of MDA-MB-231 cells.Results: 1. Western blot assay showed that E-cadherin was downregulated(p<0.05)and Slug, Vimentin were upregulated(p<0.05)with transient over expression of PRMT2, meanwhile, E-cadherin was upregulated(p<0.05)and Slug, Vimentin were downregulated(p<0.05)with knock-down of PRMT2.2. Morphological changes of MDA-MB-231 cells could be observed when the expression of PRMT2 was upregulated. Transwell invasion assay showed that the rate of invasion of +DOX group were increased(p<0.001), compared with-DOX group, when PRMT2 was over expressed, but Scratch-wound assay showed no significant difference on the rate of migration of both groups(p>0.05). Western blot assay showed the same results of transient over expression of PRMT2.3. Protein microarray results suggested that PRMT2 probably affected the invasion and migration of MDA-MB-231 cells through the Wnt/β-catenin signal pathway, and Western blot assay results showed the expression of p-GSK-3β(Ser9) was upregulated(p<0.05), and the expression of p- β-catenin was downregulated(p<0.05).Conclusions: 1. PRMT2 induces the EMT of breast cancer MDA-MB-231 cells.2. PRMT2 promotes the invasion of breast cancer MDA-MB-231 cells.3. PRMT2 could affect the invasion and migration of MDA-MB-231 cells through modulation of the phosphorylation of β-catenin.