The Effect Of Hydrogen Inhalation On Chronic Hypoxic Pulmonary Hypertention

Objective: To discuss the effect of hydrogen inhalation on chronic hypoxic pulmonary hypertention.Methods:(1)Animal grouping and hypoxic treatment: 30 healthy male mice were randomly divided into three groups: control group(Control),hypoxia group(Hypoxia),hypoxia+ hydrogen inhalation group(Hypoxia + H2). Each group had 10 mice. The control group received no treatment. Hypoxia group inhaled hypoxic gas mixture(10% O2 + 90% N2)were placed in an hyperbaric oxygen, kept 8 hours a day. The rest of the time placed in normoxic for 28 days. Hypoxia + hydrogen inhalation group adding hydrogen(10%O2+4%H2+90%N2)in hypoxic gas mixture, the other with the same set of hypoxia. Hypoxia for 28 days, took tissue samples to check;(2) Hypoxia model checking: after 28 days of hypoxia exposure to 3% pentobarbital sodium( 25mg/kg) by intraperitoneal injection, intubation, carotid artery blood, and then check routine blood, statistical three groups of RBC,HGB;(3)Assessment of pulmonary hypertension: after modeling is complete, remove the thoracic to take cardiac tissue, separating the left and right ventricles and weighed, to obtain ventricular hypertrophy index. Take the same side of the same lung segment, 4% poly formaldehyde fixed, sliced, HE staining, using image processing program6.0 measure the inside and outside diameters of the small blood vessels. By ventricular hypertrophy index and pulmonary arterial hyperplasia tiny two indirect indicators to reflect;(4) Lung damage assessment: lung tissue HE staining was observed using an optical microscope, lung pathology scores were referring Mikawa methods;(5)Measurement of inflammatory cytokines in lung tissue: homogenated lung tissue, then extracting tissue fluid, using indirect ELISA assay;(6)Measurement of MDA in lung tissue : homogenated lung tissue, then extracting tissue fluid to detective MDA.Results:(1)RBC and HGB determination: hypoxia group and hypoxia + hydrogen inhalation group of RBC,HGB were all increased, in accordance with chronic hypoxia changes of the blood, and the change of the hypoxia group in the most significant;(2)Ventricular hypertrophy index determination: compared with the control group, hypoxia group and hypoxia + hydrogen inhalation group indexes increased, in line with the pathological changes of pulmonary hypertension, but hypoxia + hydrogen inhalation group ventricular hypertrophy index is lower than that of hypoxia;(3)Lung damage assessment: hypoxia group is higher than the control group on lung pathology score. Hypoxia group has occured congestion and inflammatory cell infiltration in the lung tissue of the gap. Hypoxia + hydrogen inhalation group had no significant lung injury performance;(4)Detection of inflammatory cytokines: proinflammatory cytokine neutrophil elastase in the hypoxia group and hypoxia + hydrogen inhalation group were increased, but hypoxia + hydrogen treatment group is lesser.Conclusions: Inhalation of hydrogen through its antioxidant and anti-inflammatory role in protecting the lung tissue and improve pulmonary hypertension.