| Background and Purpose of Study Hepatic cancer is one of the common Gastrointestinal Malignant Tumor in the world. However, hepatocellular carcinoma(HCC) constitutes the majority in hepatic cancer. The hepatic cancer patients have poor integral prognosis, especially for mal patients. In the countries where the patients having hepatic cancer increase for every year in the world, China is ranked in the first place, accounting for a half of overall incidence in the world. Since the early clinical symptoms of heptatic cancer are not typical. Most of the patients have entered in advanced hepatic cancer when they were diagnosed as hepatic cancer. For this reason, they have lost the opportunities of radical treatment including hepatectomy and liver transplantation. As for the hepatic cancer patients having the opportunities of surgical radical treatment, the five-year reoccurrence rate is 50% ~ 70% after hepatectomy. The main factors which cause lowering of survival rate and life quality are postoperative metastasis and reoccurrence of hepatic cancer. Hence so as a result, in order to increase the survival rate and improve life quality, we must improve diagnostic accuracy of hepatic cancer as far as possible on the onehand and adopt anti-relapse therapy against metastasis on the other hand. mi RNA(micro RNA) is micro-molecule RNA with wide distribution and non-coding and single chain. Its length is 18-25 nt. The studies have shown that the interaction between mi RNA cancer gene and cancer suppressor gene is the one of mechanisms of malignant tumor included in HCC. mi R-422 a acted as the member of mi RNA, the study of mi R-422 a is less relatively in the related studies of tumor. The results of the study have shown that mi R-422 a has played the important roles in occurrence process of laryngeal carcinoma, mammary cancer, colorectal adeno carcinoma, intestinal-type gastric carcinoma and osteosarcoma, and the apoptosis mechanisms after craniocerebral injury(such as Ischemic-reperfusion Injury, contusion of brain). In this experiment, it is intended to apply the real-time quantitative technology to determine the expression of mi R-422 a in hepatoma carcinoma cell and hepatocellular carcinoma tissue and analyze its expression and the relationships to clinic pathological characteristics with HCC patients ages, gender, tumor size, knot quantity of tumor, tumor differentiation and venous invasion, further study deeply that mi R-422 a plays the action mechanism in occurrence and development of hepatic cancer and lay the foundation of mean so as to provide the basis for mi RNA to play the new action in hepatic cancer and meanwhile to provide the basis for biological diagnosis, prognostic judgment and treatment and also to provide the gene target fordeveloping new medicines for treatment of hepatic cancer.Methods 1.By means of q RT-PCR Technology, to determine the expression differentials of mi R-422 a in human normal hepatocytes(HL-7702) and HCC Cell Strain with different invasion ability(Hep G2<MHCC97-H<JCCLM3) 2.By means of q RT-PCR technology, to determine the expression and correlation analysis of mi R-442 a in HCC tissue and corresponding adjacent tissues in 30 cases of HCC patients and in 5 cases of normal hepatic tissue 3.To analyze the expression of mi R-422 a and corelation between HCC clinic pathological characteristicsResults 1.The determination of q RT-PCR has shown that mi R-422 a has the expression in human normal hepatocytes(HL-7702) and HCC Cell Strain with the different invasion ability(Hep G2, MHCC97-H and HCCLM3). In comparison with optional two kinds cell stains(p<0.05), the differentials have the statistical significance. The expression of mi R-422 a in four kinds of cells: HL-7702>Hep G2>MHCC97-H>HCCLM3 2.The results of q RT-PCR has shown that the expression differentials of mi R-422 a in HCC tissue, corresponding adjacent tissues and normal tissue have the statistical significance(p<0.05) and comparing with comparisonhepatic tissue, mi R-422 a has the obvious downward in HCC tissue. 3.The expression of mi R-422 a in multiple nodules of tumor, poor differentiation and HCC tissue with venous invasion is distinctly higher than HCC tissue of the single nodule(p = 0.036), high-middle differentiation(p=0.035), non venous invasion(p = 0.0016). The expression of mi R-422 a has not obvious correlation with gender, age, diameter of tumor, AFP value, liver cirrhosis and peplos.Conclusion 1.mi R-422 a expresses upward in human normal hepatocytes, expresses downward in HCC cell strain and expresses obvious downward as HCC cell invasion and transfer increases. 2.The expression of mi R-422 a apparently decreases in HCC tissue and has the relationships to clinic pathological characteristics of HCC patients in multiple nodules of tumor, poor differentiation and accompanying venous invasion. Prompting that it may become a new biological marker of early diagnosis and prognosis assessment... |
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