Research On The Relationship Between Levels Changes Of Circulating MiRNAs And The Early Recurrence After Radiofrequency Ablation In Non-valvular Atrial Fibrillation Patients

Objective In this study, we monitored early recurrence after radiofrequency current ablation by detecting levels of circulating muscle-specific mir-1, mir-21,mir-133 b.Method 15 patients underwent radiofrequency ablation operation were included in our study, including 9 males and 6 females, average age(58.80± 14.75y). Peripheral vein blood was drawn from patients before the operation, the 24 h, 48 h, 72 h after operation respectively. Serum from peripheral blood was obtained for cardiac troponin T(c Tn T) measurement. Plasma was centrifuged from peripheral blood for measuring mir-1, mir-21, mir-133 b by quantitative reverse transcription polymerase chain reaction(q RT-PCR). Correlations between mi RNAs and c Tn T were analyzed. Follow-up 3 months, Correlations between mi RNAs, c Tn T and early recurrence were analyzed.Result There were uncomplicated in all the patients enrolled. The levels of c Tn T, circulating mir-1, mir-21, mir-133 b were elevated to peak at 24 h after RFCA. After that the expression levels of all three mi RNAs and c Tn T in plasma declined gradually from 24 h to 72 h. However, c Tn T was still higer than those in controls. There was no significant correlations between circulating mi RNAs and c Tn T. Follow-up 3 months, three cases of patients recurrenced after RFCA, including 1 patients with paroxysmal atrial fibrillation, 2 patients with persistent atrial fibrillation. 12 patients without recurrence within 3 months after operation, including 11 patients with paroxysmal atrial fibrillation, 1 patients with persistent atrial fibrillation. Compared recurrent group with no recurrence group, mir-1 peak was significantly increased, the operation time was significantly prolonged. There was no significant difference between the two groups in c Tn T, circulating mi RNAs at 48 h, 72 h after RFCA.Conclusion The dynamic change in circulating muscle-specific mi RNAs can relect myocardial injury after radiofrequency catheter ablation. Long duration of ablation correlated well with early recurrence of AF after radiofrequency ablation. Circulating mir-1 can be used as a marker for early recurrence after radiofrequency catheter ablation.