Effects Of Acupuncture Combined With Treadmill Training On The Expression Of A2a Adenosine Receptors And IL-12 In Brain Tissues Of EAE Mice

Objective:Through the observation of acupuncture combined with the effects of treadmill training on brain tissue of EAE mice of A2a adenosine receptor and expression of IL-12 on serum, to investigate the effect of acupuncture combined with treadmill training on EAE mice and its mechanism.Methods:A total of 60 C57BL/6 mice were randomly divided into control group, model group, acupuncture group, acupuncture combined with treadmill training group and training group. With MOG35-55 and complete Freund’s adjuvant (CFA) to produce the immune emulsion, induced EAE model, adjuvant control group with the same amount of PBS instead of MOG35-55. The model group and adjuvant control group not given any treatment, acupuncture group was given acupuncture Baihui, treadmill group were given treadmill training, acupuncture combined with treadmill training group was given acupuncture Baihui acupoint combined with treadmill training. Immune day record for 0 days, and on the day of PT injection immunization and second days. From the eleventh day after immunization to begin treatment.6 days per week treatment. the changes of body weight were observed daily until twenty-eight days after immunization.Results:1.The body weight and the changes of clinical symptoms:Compared with the adjuvant control group, model group, acupuncture group, acupuncture combined with treadmill training group, treadmill training group had significant difference (P<0.05); compared with the model group, acupuncture group, acupuncture combined with treadmill training group, treadmill training group had significant difference (P<0.05); acupuncture combined with treadmill training group, with simple acupuncture group compared with treadmill training group had significant difference (P<0.05), was statistically significant.2. The expression of IL-12 in serum:Compared with the adjuvant control group, model group, acupuncture group, acupuncture combined with treadmill training group, IL-12 treadmill training group serum concentration had significant difference (P<0.05), was statistically significant; compared with the model group, acupuncture group, acupuncture combined with treadmill training group, treadmill training group had significant difference (P<0.05), there was statistically significant difference; acupuncture combined with treadmill training group, with simple acupuncture group compared with treadmill training group had significant difference (P<0.05), was statistically significant, different3. The expression of A2aR in brain tissue A2aR content:Compared with adjuvant group, model group, acupuncture group, running group, acupuncture combined with adenosine A2a receptor positive cells within the visual range of the number of treadmill training group in the brain of mice had significant difference (P<0.05), was statistically significant; compared with the model group, acupuncture group, treadmill training group, acupuncture combined with treadmill the training group in brain tissue within the visual range of the A2aR number had significant difference (P<0.05), was statistically significant; acupuncture combined with treadmill training group, with simple acupuncture group compared with treadmill training group had significant difference (P<0.05), compared with statistical significanceConclusion:1. Acupuncture, treadmill training, acupuncture combined with treadmill training can reduce the neurological function of EAE mice. The acupuncture combined with treadmill training group is better than the acupuncture group and treadmill training group.2. Acupuncture, treadmill training, acupuncture combined with treadmill training can inhibit the expression of pro-inflammatory cytokines IL-12 in EAE mice. The acupuncture combined with treadmill training group is better than the acupuncture group and treadmill training group.3. Acupuncture, treadmill training, acupuncture combined with treadmill training can promote the expression of A2a adenosine receptors in brain tissues of EAE mice. The acupuncture combined with treadmill training group is better than the acupuncture group and treadmill training group.