Studies On The Interaction Of Multi-Components In Tongmai Compound

This project was focusd on Puerarin(Pur),Salvianolic acid B(SalB) and Ferulic acid(FA),which were the active ingredients of Tongmai compound.In this paper,Caco-2 cell model and pharmacokinetic were used to optimize the proportion and study the interaction.In this study,an analytical method based on high performance liquid chromatography(HPLC) had been established to determine the active ingredients in traditional Chinese medicine.The results showed that the content of Pur in Radix Puerariae was 3.75%,the content of Sal B in Salvia miltiorrhiza Bge was 3.0% and the content of FA in Ligusticum chuanxiong Hort was 0.17%.According to the transport of Pur,Sal B and FA in Caco-2 cell model, the optimized formulation was determined by the central composite design-response surface methodology.The results showed that the optimized proportion of Pur,Sal B and FA was 20.6:22.5:1.0,when three active ingredients were absorbed best.The characteristics of the uptake of Pur,SalB and FA was investigated.The effects of time,drug concentration,pH,P-gp,MRP and compatibility on their uptake were observed.The results showed that the uptake of Pur and FA in Caco-2 cell was a passive diffusion process,and active transportation was also involved.P-gp and MRP played an important role in the uptake of Pur and FA. Pur and FA could promte the absorption of each other.The uptake of SalB in Caco-2 cell was a passive diffusion process.P-gp and MRP had no effect on the uptake of SalB. SalB to had no influence on the uptake of Pur and Ferulic.Studied on the pharmacokinetic comparisons of Pur,SalB and FA after oral administration of monomer and combination.The results indicated that three active ingredients in rats could be described by a two-compartment model.Compatibility can promote the absorption of puerarin,Sal B and ferulic acid and slow the elimination of Sal B and ferulic acid.The optimal compatibility made more contribution to efficacy than the original compatibility.