Clinicopathological Factors And The Expression Of Kiss-1 MMP-9 Analysis In Liver Metastases Of Colorectal Cancer

Colorectal cancer(CRC) is one of the most common cancer in the world. According to the study of 2012, CRC ranks second in male cancers(9%), while the third in female(8%).Because the early symptoms were not obvious and difficult to be detected, most patients often the first to treat while the distant metastasis had been occurred; And the prognosis always would be poor. The distant metastasis of the cancer is an complex,multifactorial, multi-step participation result. To explore the molecular mechanisms of metastasis and the relevance between clinicopathological factors and its prognosis can help early detection, early diagnosis and early treatment.This article is divided into two parts:(1) To analysis the relerationship between clinicopathological factors with liver metastasis and the prognosisof the colorectal cancer patients. (2) To analysis the expression of Kiss-1、MMP-9 in normal mucosa, colorectal cancer and simultaneous liver metastases of colorectal cancer and its relevance with the patients’ prognosis.Part 1 liver metastasis and prognosis analysis of the clinicopathological factors in colorectal cancer patientsThe early symptoms of colorectal cancer patients were not obvious and difficult to be detected, most patients in the first time to treat while the distant metastasis had been occurred and they always had a poor outcome.To analysis 275 cases of colorectal cancer by SPSS 19.0 statistical software,we explore the relationship between liver metastasis and clinicopathological factors in order to achieve early diagnosis and treatment;At the same time to detect the recurrence and the metastasis by assessing the prognosis in order to prolong the disease-free survival and overall survival time and to improve the quality of the patients’ life.Part 2 To analysis the expression of Kiss-1、MMP-9 in normal mucosa, colorectal cancer and SCLM and its relevance with the patients’ prognosisMetastasis is not only the characteristic of malignant tumors,but also the main factor to affect the patients’ prognosis. With the more in-depth discussion of the molecular mechanism of the development, invasion,metastasis of the tumor and the completion of the Human Genome Project,Oncogenes(metastasis suppressor genes,metastasis-promoting gene),tumor suppressor geneare gradually accepted by the majority of scholars. Kiss-1 gene as a tumor metastasis suppressor gene has been extensively studied,as well as MMP-9 gene. But the expression of Kiss-1 and MMP-9 in SCLM has not been reported yet. We would detect the expression of Kiss-1 and MMP-9 in 96 cases of SCLM,69 cases of CRC, 50 cases of the normal instestinal spacimens by immunohistochemical DAB technique and explore its relationship with the progression and prognosis, and analysis the expression correlation between Kiss-1 and MMP-9 in SCLM.Conclusion1、Liver metastases of colorectal cancer has relationship with tumor differentiation, intestinal tumor location, lymph node metastasis, fecal occult blood, ascites, serum CEA, CA199 levels and HBV, while has nothing with gender, age, tumor size. Prognosis of liver metastases of colorectal cancer patients is related with tumor differentiation, lymph node metastasis, postoperative chemotherapy standardization, ascites, serum CEA, CA199 levels and HBV; Further more, tumor differentiation, Standardized postoperative chemotherapy and HBV are independent prognostic factors in prognosis.2、The positive expression rates of Kiss-1 and MMP-9 among the three tissues were significant difference. The positive expression rates of Kiss-1 and MMP-9 were associated with tumor invasion depth, lymph node metastasis and the prognosis.The expression of Kiss-1 and MMP-9 in SCLM was negatively associated.