Role Of MiRNA-34a And YY1 In Radon Induced Apoptosis Of BEAS-2B Cells

Objective: To observe the role of mi RNA-34 a and YY1 in apoptosis of BEAS-2B cells induced by radon to provide experimental data for mechanistic study.Methods: BEAS-2B cells in good condition were trypsinized and planted onto Transwell membrane. 12 hours later, the cells were directly exposed to radon at the concentration of 20000Bq/m3 for 30 mins twice as 1st generation(Rn1), and continued to conduct for Rn5, Rn10, Rn15, and Rn20. The apoptosis rate of the cells was determined by flow cytometry. The expression levels of miR-34 a were detected by Real-time PCR. The protein levels of YY1, Bcl-2, PARP-1 and Bax were determined by Western blot. Bioinformatic technology was used to predict the target gene of miR-34 a, and PcDNA3.1-YY1 and shRNA-YY1 were transfected into the cells individually to construct over express and silent carrier of YY1.Results:(1) The radon exposure resulted in an increase in cell apoptosis and miR-34 a expression, and a decrease in YY1 expression;(2) Transfection with miR-34 a promoted the apoptosis rate in BEAS-2B cells, with down-regulation of Bcl-2 and PARP-1, and up-regulation of Bax;(3) Over expression of YY1 suppressed the apoptosis rate in BEAS-2B cells, with down-regulation of Bax and up-regulation of Bcl-2 and PARP-1.Conclusions: The results indicated a negative regulation of miR-34 a on YY1 expression, which upregulates Bax and downregulates Bcl-2 and PARP-1expression, and leads to an increase in apoptosis of BEAS-2B cells upon radon exposure. However, the detailed molecular mechanism needs further investigation.